Introduction to Single Cell Sequencing
Single Cell Sequencing reveals the full complexity of cellular diversity compared to bulk sequencing. It is primarily employed to solve complications related to the study of unusual cell types, cell-lineage associations, and samples of heterogeneous nature. By doing the in-depth single-cell sequencing, the cell functions of individual cells can be explored easily and efficiently.
By combining the 10x Genomics Chromium system and Illumina NGS platform, Novogene offers high-quality end-to-end single cell sequencing solutions and services, including Single Cell Gene Expression, Single Cell Immune Profiling, and Single Cell ATAC, etc. As a Certified Service Provider of 10x Genomics, Novogene provides efficient and accurate support for your research.
Applications of Single Cell Sequencing
- Single Cell Gene Expression: Profiling of cellular heterogeneity, novel targets, and biomarker in each cell from a heterogeneous cell population.
- Single Cell Immune Profiling: Analyze full-length, paired B-cell or T-cell receptors, antigen specificity, and gene expression, all from a single cell
Benefits of Single Cell Sequencing
- Single-cell sequencing intends to unveil the diversity of the transcriptome in heterogeneous cell samples. It provides deeper insights into 3’ and 5’ gene expression and characterizes cell differences, at a single-cell resolution.
- Novogene provides an end-to-end package of single-cell sequencing solutions making use of the latest technology on the market: 10x Genomics Chromium system and Illumina NGS platform. We offer high throughput and high accuracy single-cell sequencing service, coupled with a fast turnaround time.
10x Single Cell Sequencing Specifications: Sample Requirements
| Single cells suspension | |
| Single nuclei suspension |
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10x Single Cell RNA Sequencing Specifications: Sequencing & Analysis
| Sequencing Platform | Illumina NovaSeq 6000 Sequencing System |
| Read length | Paired-end 150bp |
| Recommended Data Amount | ~150G raw data per sample |
| Content of Data Analysis |
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Novogene Workflow of 10x Single Cell Sequencing Service
From sample preparation, library preparation, sequencing and data quality control, to bioinformatics analysis, Novogene provides high-quality products and professional services. Each step is performed in agreement with a high scientific standard and meticulous design to ensure high-quality research results.
Featured publications of 10x Single Cell Sequencing
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Journal: CellIssue Date: Oct 15，2020IF: 31.398DOI: https://doi.org/10.1016/j.cell.2020.08.040
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Journal: Journal of HepatologyIssue date: Jun 22， 2021IF: 25.083DOI: https://doi.org/10.1016/j.jhep.2021.06.023
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Journal: Nature CommunicationsIssue date:Jun 23, 2021IF:14.919DOI: https://doi.org/10.1038/s41467-021-24213-6
Demo Results
Figure 1 The t-SNE and UMAP plots
Note:Each point represents a cell, and each color designates a cluster.
For visualization purposes, dimensionality was further reduced to 2D using t-distributed stochastic neighbor embedding (t-SNE) and uniform manifold approximation and projection (UMAP). Both of them try to find a low-dimensional representation that preserves relationships between neighbors in high-dimensional space. Compared to t-SNE, the UMAP visualization tends to have more compact visual clusters with more empty spaces between them.
Figure 2 Expression Pattern of Marker Genes
Note: Darker red represents a higher expression level, and darker blue dictates a lower expression level.
Heatmaps show the expression of indicated marker genes for given cells and features. In this case, we plotted the top 10 markers (or all markers if less than 10) for each cluster.
Figure 3 Expression Level of Marker Genes
Note: The X-axis represents the cluster, and the Y-axis represents the expression level.
Violin plots show the relative expression levels of marker genes among all clusters. (Only six marker genes are shown here)
