Small RNAs (sRNAs) are short RNA molecules, usually non-coding, involved with gene silencing and the post-transcriptional regulation of gene expression. sRNA Sequencing (sRNA-seq) is a method that enables the in-depth investigation of these RNAs, in special microRNAs (miRNAs, 18-40nt in length).
Novogene sRNA-seq service is an effective approach to selectively target any species of sRNAs with unprecedented sensitivity and high resolution, all in a single analysis. Coupled with a robust in-house bioinformatics pipeline, Novogene sRNA-seq service has been assisting researchers to describe the differential expression of miRNAs, structural alterations, and to discover novel sRNAs.
sRNA-seq has been supporting many compelling research avenues, including:
Note: Sample amounts are listed for reference only. Download the Service Specifications or Sample Requirements to learn more. For detailed information, please contact us with your customized inquiry.
Note: Sequencing depths and analysis contents displayed are for reference only. Download the Service Specifications to learn more. For detailed information, please contact us.
The project workflow starts with sample quality control (Sample QC) to ensure that your samples meet the criteria of the sRNA-Seq technique. Then, the appropriate library is prepared according to your target organism and application, and subsequently tested for its quality (Library QC). Next, a single-end 50 bp sequencing strategy is used to sequence the samples and the resulting data is also checked for its quality (Data QC). Finally, bioinformatic analyses are performed and publication-ready results are provided.
Novogene’s comprehensive sRNA-seq empowers researchers to investigate the differential expression and regulatory network of miRNAs, structural alterations, and to discover novel small RNAs. Here is a list of publications below that have used Novogene’s sRNA-seq services.
A genomic and epigenomic atlas of prostate cancer in Asian populations
NatureIssue Date: 25 March 2020IF: 42.778DOI: 10.1038/s41586-020-2135-x
Spliceosome disassembly factors ILP1 and NTR1 promote miRNA biogenesis in Arabidopsis thaliana
Nucleic Acids ResearchIssue Date: 19 June 2019IF: 11.501DOI: 10.1093/nar/gkz526
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