16S/18S/ITS Amplicon Metagenomic Sequencing is an ultra-deep DNA sequencing method that focuses on sequencing specific target regions (amplicons). Short (<500 bp) hypervariable regions of conserved genes or intergenic regions are amplified by PCR and analyzed by next generation sequencing (NGS) technology, to identify and differentiate multiple microbial species from complicated samples. Amplicon metagenomic sequencing is designated to sequence the target genes of 16S ribosomal RNA (rRNA), or 18S rRNA and Internal Transcribed Spacer (ITS) rRNA by universal primers, to describe and compare the phylogeny and taxonomy of bacteria (and archaea) and fungi (such as yeasts, molds and etc.), respectively.
Novogene amplicon metagenomic sequencing services provide to efficiently screen for variants and target organisms, and describe and compare the diversity of multiple complex environments. The approach is frequently used in population and community microbial ecology studies, phylogenetic reconstruction of target microbial groups, identification of individual species in pure cultures, and detection of organisms of interest (pathogens or beneficial), among many others.
Amplicon metagenomic sequencing is an effective way to investigate all microorganisms present in diverse samples, including:
Amplicon metagenomic sequencing is considerably simpler, faster and more efficient, compared with the whole microbial genome sequencing by separating each single species in the samples. Based on its highly targeted characteristics and multiplexing, information on thousands of amplicons is obtained by specific amplification in a single run without host contamination. High coverage is achieved, and resequencing is possible even in GC-rich regions.
Novogene has rich experience with amplicon projects and recently developed new pipelines to sequence amplicons using both short reads and long reads (full-length) technology for 16S and 18S amplicons. At Novogene, Amplicon metagenomic sequencing services can help retain a great level of flexibility in the experimental design to get analysis and clustering by both “OTU” (operational taxonomic units) and ASV (DADA2) pipelines. Customized analysis can also be available on the online Qiime 2 platform at will.
Note: Sample amounts displayed are for reference only. Download the Service Specifications to learn more. For detailed information, please contact us.
Note: Analysis contents displayed are for reference only. Download the Service Specifications to learn more. For detailed information, please contact us with your customized requests.
The workflow for a new project begins with sample preparation and subsequent quality control. Once the quality of the DNA sample has been validated, PCR amplification will be performed on target regions (amplicons). The amplicons are then purified for library preparation, after which, library quality control commences. Sequencing by synthesis (SBS) technology is used by the Illumina NovaSeq 6000 platform to sequence the library and, once sequencing is complete and data quality control is performed, bioinformatics analysis is conducted to obtain high-quality results and create publication-ready figures.
Amplicon Metagenomic Sequencing is a powerful tool for researchers aiming at the identification and differentiation of microbial species, either using OTUs or ASVs. With 16S/18S/ITS rRNA sequencing results, the microbial diversity of the environment through the Alpha (α) and Beta (β) diversity analysis can be characterized, to identify, describe, and track microorganisms of interest. Here is a list of featured publications that have used Novogene Amplicon Metagenomic Sequencing services.
Drosophila Histone Demethylase KDM5 Regulates Social Behavior through Immune Control and Gut Microbiota Maintenance
Cell Host & MicrobeIssue Date: 10 April 2019IF: 15.923DOI: 10.1016/j.chom.2019.02.003
Evaluating the impact of bamboo biochar on the fungal community succession during chicken manure composting
Bioresource TechnologyIssue Date: January 2019IF: 7.539DOI: 10.1016/j.biortech.2018.10.045
Gut-dependent microbial translocation induces inflammation and cardiovascular events after ST-elevation myocardial infarction
MicrobiomeIssue Date: 03 April 2018IF: 11.607DOI: 10.1186/s40168-018-0441-4
The microbiota maintains homeostasis of liver-resident γδT-17 cells in a lipid antigen/CD1d-dependent manner
Nature CommunicationsIssue Date: 09 January 2017IF: 12.121DOI: 10.1038/ncomms13839
Gut microbiota dysbiosis contributes to the development of hypertension
MicrobiomeIssue Date: 01 February 2017IF: 11.607DOI: 10.1186/s40168-016-0222-x
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