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DNA methylation at the C5 position of cytosine plays a crucial role in gene expression and chromatin remodeling. Perturbations in methylation patterns are associated with tumorigenesis, neurodegenerative diseases and neurological disorders. Bioinformatic analysis of methylomes is widely used in various research areas, including studies on gene regulation, cell differentiation, embryogenesis, aging, occurrence and development of disease, phenotypic diversity and evolution in plants and animals.
Novogene provides whole genome bisulfite sequencing (WGBS) to efficiently identify methylated cytosines on a genome-wide scale with a single nucleotide resolution. The deep reach of the methylome is imperative for understanding gene expression and multiple biological processes that are subject to epigenetic regulation.
Novogene delivers high-quality data and publication-ready analysis results of WGBS services, to faciliate research including:
Note: Sample amounts are listed for reference only. Download the Service Specifications to learn more. For detailed information, please contact us with your customized requests.
Note: Sequencing parameters and analysis contents displayed are only for reference. Download the Service Specifications to learn more. For detailed information, please contact us with your customized requests.
The Novogene WGBS service is comprised of four steps. Sample preparation is followed by library preparation, sequencing and bioinformatics analysis. To construct a methylation library, DNA fragmentation is performed initially, and sodium bisulfite converts the unmethylated cytosines into uracils. Libraries are then sequenced using Illumina PE150 and the reads are processed with bioinformatics pipelines.
In order to ensure the accuracy and reliability of sequencing data, Novogene audits every experimental step strictly by quality control and ensures high-quality data output fundamentally, from DNA sampling to obtaining the final data report. Obtaining high-quality data is the premise to ensure that bioinformatics analysis is correct, comprehensive and credible.
Whole Genome Bisulfite Sequencing (WGBS) is a powerful method to identify individually methylated cytosines on a genome-wide scale. The deep reach of the methylome (including methylated cytosine detection), the promoter detection of differentially methylated sites and regions, and related functional analysis of the Differentially Methylated Region (DMR) and Differentially Methylated Promoter (DMP), are all essential for understanding gene expression and other processes that are subject to epigenetic regulation. Here summarizes a list of publications that used Novogene WGBS service.
PRC2-AgeIndex as a universal biomarker of aging and rejuvenation
Nature CommunicationsIssue Date: 16 July 2024IF: 14.7DOI: 10.1038/s41467-024-50098-2
Mutant IDH1 inhibition induces dsDNA sensing to activate tumor immunity
Science Issue Date: 6 July 2024IF: 44.7DOI: 10.1126/science.adl6173
Epigenetic and transcriptional consequences in the endosperm of chemically induced transposon mobilization in Arabidopsis
Nucleic Acids Research Issue Date: 5 July 2024IF: 16.6DOI: 10.1093/nar/gkae572
A conserved Pol II elongator SPT6L mediates Pol V transcription to regulate RNA-directed DNA methylation in Arabidopsis
Nature CommunicationsIssue Date: 25 May 2024IF: 14.7DOI: 10.1038/s41467-024-48940-8
Auto-suppression of Tet dioxygenases protects the mouse oocyte genome from oxidative demethylation
Nature Structural & Molecular BiologyIssue Date: 4 January 2024IF: 16.8DOI: 10.1038/s41594-023-01125-1
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