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Circular RNA Sequencing (circRNA-seq)

Introduction to Circular RNA Sequencing

Circular RNA (circRNA) is a highly stable molecule of ncRNA, in form of a covalently closed loop that lacks the 5’end caps and the 3’ poly(A) tails. The circular structure grants circRNAs resistance against exonuclease digestion, a characteristic that can be exploited in library construction.

Novogene’s circRNA sequencing service (circRNA-seq) uses next-generation sequencing (NGS) technology to support a wide range of investigations focused on circRNA. The regulatory function of circRNAs may be involved in many biological processes, such as regulation of gene expression by acting as miRNA “sponges”, transport of miRNAs, and overall regulation of protein synthesis.

Applications of Circular RNA sequencing

circRNA-seq reveals the molecular importance in regulating gene expression including:

  • Profiling known and novel circRNAs and prediction of target genes
  • Identification of biomarkers for diagnostics and classification of cancer or diseases
  • Exploration of regulatory networks between ncRNA and miRNA
  • Understanding the mechanisms of tissue or organism development based on transcript profiles

Benefits of Novogene circRNA sequencing

  • The circRNA-seq service relies on Novogene’s extensive experience supporting many investigators in research. This service offers a comprehensive analysis performed with industry-standard software and a mature in-house bioinformatics pipeline. Novogene provides a convenient and fast solution to assist researchers in understanding the regulatory networks and the deep regulatory mechanisms of gene expression.

circRNA-seq Specifications: RNA Sample Requirements

Library Type Sample Type Amount RNA Integrity Number (Agilent 2100) Purity (NanoDrop)
circRNA Library
(rRNA and linear
RNA depletion)
Total RNA ≥ 2 μg Animal ≥ 7, Plant ≥ 6.5, with smooth baseline OD260/280 ≥ 2.0;
OD260/230 ≥ 2.0;
no degradation,
no contamination

Note: Sample amounts displayed are for reference only. Download the Service Specifications or Sample Requirements learn more. For detailed information, please contact us.

circRNA-seq Specifications: Sequencing and Analysis

Sequencing Platform Illumina NovaSeq 6000
Read Length Paired-end 150bp
Recommended Sequencing Depth ≥ 40 million read pair per sample
Standard Data Analysis
  • Data Quality Control
  • circRNA Identification
  • Transcript Expression Quantification & Differential Expression Analysis
  • Functional Enrichment Analysis
  • CircRNA Target Gene Prediction
  • Note: Recommended data outputs and analysis contents are displayed for reference only. Download the Service Specifications to learn more. For detailed information, please contact us.

    Novogene circRNA Sequencing Project Workflow

    The workflow begins with the evaluation of sample quality. After ribosomal RNA (rRNA) depletion, samples are digested by RNase R to remove linear transcripts. Strand-specific libraries (also known as stranded/directional libraries) are prepared, and the sequencing is performed using a paired-end 150bp strategy. Quality assessment of the samples, libraries, and sequencing data are performed to guarantee high-quality results. Bioinformatics pipelines can be tailored to multiple needs, providing high-quality, publication-ready results.

    Featured publications using Novogene circRNA service

    Novogene’s circRNAs service helps researchers to understand regulation mechanisms of gene expression through exciting and new research avenues. There is a summarized list of publications below that have used Novogene’s circRNA-seq services.

    Length Distribution of circRNA


    Differential Expression

    GO Enrichment

    KEGG Enrichment