Compared to bulk RNA-seq, which shows an average gene expression level among a pool of cells, single-cell RNA-seq, based on the 10x Chromium platform, can reveal cell-to-cell differences and cellular heterogeneities that are often obscured by bulk RNA-seq. Single-cell RNA-seq has been widely applied to multiple research areas, including immunology, neurology, and stem-cell biology, etc.

Learning Objectives:

• The entire pipeline of 10x single-cell RNA sequencing
• Explore the transcriptome and conduct immune profiling on a cell by cell basis with better clarity