Circular RNA (circRNA) is a highly stable molecule of ncRNA, in form of a covalently closed loop that lacks the 5’end caps and the 3’ poly(A) tails. The circular structure grants circRNAs resistance against exonuclease digestion, a characteristic that can be exploited in library construction.
Novogene’s circRNA sequencing service (circRNA-seq) uses next-generation sequencing (NGS) technology to support a wide range of investigations focused on circRNA. The regulatory function of circRNAs may be involved in many biological processes, such as regulation of gene expression by acting as miRNA “sponges”, transport of miRNAs, and overall regulation of protein synthesis.
circRNA-seq reveals the molecular importance in regulating gene expression including:
Note: Sample amounts displayed are for reference only. Download the Service Specifications or Sample Requirements learn more. For detailed information, please contact us.
Note: Recommended data outputs and analysis contents are displayed for reference only. Download the Service Specifications to learn more. For detailed information, please contact us.
The workflow begins with the evaluation of sample quality. After ribosomal RNA (rRNA) depletion, samples are digested by RNase R to remove linear transcripts. Strand-specific libraries (also known as stranded/directional libraries) are prepared, and the sequencing is performed using a paired-end 150bp strategy. Quality assessment of the samples, libraries, and sequencing data are performed to guarantee high-quality results. Bioinformatics pipelines can be tailored to multiple needs, providing high-quality, publication-ready results.
Novogene’s circRNAs service helps researchers to understand regulation mechanisms of gene expression through exciting and new research avenues. There is a summarized list of publications below that have used Novogene’s circRNA-seq services.
Circular RNA circCORO1C promotes laryngeal squamous cell carcinoma progression by modulating the let-7c-5p/PBX3 axis
Molecular CancerIssue Date: 02 June 2020IF: 15.302DOI: 10.1186/s12943-020-01215-4
A circRNA signature predicts postoperative recurrence in stage II/III colon cancer
EMBO Molecular MedicineIssue Date: 2 September 2019IF: 8.821DOI: 10.15252/emmm.201810168
The field is required.