Product introduction
Lipids are a class of bioorganic molecules that are insoluble in water and easily soluble in non-polar solvents. They are involved in a variety of life processes and closely related to the occurrence and development of a variety of diseases. Lipidomics is a high-throughput analytical technique based on liquid chromatography-mass spectrometry (LC-MS) to systematically analyze and understand the nature and functions of lipids by conducting comprehensive and systematic analysis and identification of lipids in organisms, tissues, or cells.
Quantitative lipidomics is a high-throughput targeted technique by performing MRM mode that enables accurate lipid quantification.
Function and classification of lipids:
Lipids function: Lipids are essential components of biological systems, participating in energy metabolism, biomembrane structural organization, signal transduction, inflammatory regulation, macromolecular recognition and cellular processes including apoptosis, growth, differentiation, and motility.
Lipids classification:
- Fatty Acyls (FA)
- Glycerophospholipids (GP)
- Sterol Lipids (ST)
- Saccharolipids (SL)
- Glycerolipids (GL)
- Sphingolipids (SP)
- Prenol Lipids (PR)
- Polyketides (PK)
Advantages:
- Ultra-high Accuracy and Sensitivity
- The use of an extremely accurate mass spectrometer, the AB SCIEX6500+, allowing detection down to pg levels.
- Absolute quantitative lipid analysis and lipid quantification utilizing the internal standard approach provides more precise results (standard + isotope internal standard).
- High throughput and wide coverage
- Over 4000 and more than 51 types of lipids can be simultaneously measured in a single lipid detection process
- High reproducibility
- Enhanced reproducibility is achieved through intra-batch correction in large-scale sample cohorts.
Sample Requirements for Biomedical Metabolomics Sample：
| Sample Type | Sample | Recommended Sample | Biological Replicate |
| Liquid | Plasma, Serum, Hemolymph, Whole Blood, Milk, Egg White | 100 μL | Human≥30, Animal≥8, Plant≥3 |
| Cerebrospinal Fluid, Interstitial Fluid, Uterine Fluid, Pancreatic Juice, Bile, Pleural Effusion, Follicular Fluid, Postmortem Fluid, Tissue Fluid, Culture Medium (liquid), Culture Supernatant, Tears, Aqueous Humor, Digestive Juices,Bone Marrow (liquid) | 100 μL | ||
| Seminal Plasma, Amniotic Fluid, Prostatic Fluid, Rumen Fluid, Respiratory Condensate, Gastric Lavage Fluid, Broncho-alveolar Lavage Fluid (BALF), Urine, Sweat, Saliva, Sputum | 500 μL | ||
| Tissue | Animal Tissues, Placenta, Blood Clot, Mycelium, Nematode, Zebrafish (whole fish), Bone Marrow (solid), Nail | 100 mg | |
| Whole Insect Body, Wings (of insects), Pupa, Eggs, Large Fungi (mushroom types), Large Algae (red algae), Large Amount of Mycelium/Mycelial Balls, Cartilage, Bone (solid) | 500 mg | ||
| Zebrafish Organs, Insect Organs, Whole Microinsect Body (e.g., Drosophila) | 20 units | ||
| Solid | Feces, Intestinal Contents, Lyophilized Fecal Powder | 200 mg | |
| Milk Powder, Microbial Fermentation Product (solid), Culture Medium (solid), Earwax, Lyophilized Tissue Powder,Feed, Egg Yolk, Lyophilized Plant Powder, Lyophilized Egg Powder | 100 mg | ||
| Honey, Nasal Mucus, Sputum | 100 mg | ||
| Sludge, Soil | 600 mg | ||
| Cell | Adherent Cells, Animal Cell Lines | 1*10^7 cells | |
| E. Coli, Yeast Cells | 1×10^10 cells | ||
| Small Amount of Fungal Mycelial Balls/Mycelium, Unicellular Algae (Cyanobacteria), Large Quantities of BacterialHyphae (sediment), Mucilaginous Protoplasmic Clusters (hyphae) | 100 mg | ||
| Organelle | Lysosomes, Mitochondria, Endoplasmic Reticulum | 4×10^7 cells | |
| Exosomes, Extracellular Vesicles | 2×10^9Particles | ||
| Special Sample | Skin Tape or Patch | 2 pieces | |
| Test Strips | 2 pieces | ||
| Swab | 1 piece |
Notes:
1. Not applicable for GC-MS.
2. The amounts shown in the table are for a single round of metabolite extraction. If the sample volume is sufficient, it is recommended to provide enough for two rounds of extraction. This way, if the first extraction does not yield ideal results, it can help avoid the delay caused by having to re-submit samples.
3. Appropriate for Untargeted Metabolomics, Untargeted Plus Metabolomics, TM Widely-Targeted Metabolomics, Quantitative Lipidomics, Energy Metabolism, Amino Acids, Tryptophan, and Short-Chain Fatty Acids studies.
Sample Requirements for Plant Metabolomics Sample：
| Sample Type | Sample | Recommended Sample | Biological Replicate |
| Tissue | Root, Stem, Leaf, Fruit, Flower, Bud, Node, Callus | 600 mg | Human≥30, Animal≥8, Plant≥3 |
| Fruit Peel, Seed Coat | 600 mg | ||
| Anther, Filament, Pollen, Style, Stigma, Embryo | 1.2 g | ||
| Liquid | Root Exudate | 10 ml | |
| Juice, Wine, Fermentation Broth | 5 ml | ||
| Oils, Essential Oils, Honey, Nectar, Paste | 500 μl | ||
| Herb Extract, Herb Decoction, Plant Tissue Fluid | 500 μl | ||
| Cell | Cultured Plant Cell | 1×10^7 cells | |
| Algae | 300 mg | ||
| Solid | Lyophilized Plant Powder | 200 mg | |
| Sludge, Soil | 600 mg |
Notes:
1. Not applicable for GC-MS.
2. For extracted metabolites, please advise on the recommended quantity for the extraction process.
3. Suitable for Untargeted Metabolomics, Widely-Targeted Metabolomics, Quantitative Lipidomics, Flavonoids, Carotenoid, Anthocyanin, Energy Metabolism, Amino Acids, Short-Chain Fatty Acids Metabolomics for Plants.
4. Recommended sampling approach:
– Collect samples from 3 or more individuals for each biological replicate.
– Ensure a minimum of 3 biological replicates per sample group.
– Example: Combine leaves from 3 individual plants as one replicate; repeat for 2 more replicates (involving 6 additional individual plants) to achieve
3 biological replicates for one experimental group (e.g., Control).
– Repeat the process with 9 individual plants for the second experimental group (e.g., Treatment).
Specifications:
Project Workflow
Quantitative lipidomics research experimental procedures can be divided into sample collection, lipid extraction, LC-MS/MS detection and data analysis. The experimental flow chart is showed below. At Novogene, we strictly control each experimental step to realize the standardized operation of lipidomics, fundamentally ensure the accuracy and reliability of the detection data, and ensure the output of high-quality data.
BI analysis
