Introduction to Pre-made Library Sequencing
With our world-leading, powerful, and sophisticated sequencing platforms (Illumina, Pacbio, and Oxford Nanopore), we provide sequencing services for pre-made libraries at different read lengths and capacities to suit any cost, project scale, and turnaround.
Novogene accepts customers’ prepared libraries for sequencing. We have a variety of sequencing platforms that offer an assortment of sequencing read length, flux, and time cycle options. We directly sequence qualified libraries provided by customers and produce high-quality sequencing data, thus providing data basis for bioinformatics analysis by customers. Novogene has a professional and efficient operations team to ensure customer project cycle and data quality. We strive to provide customers with fast and easy sequencing services. Our services combine scientific scheme design, strict quality control management, rich project experience and high-quality project service to ensure that every link can be excellently completed to facilitate scientific research. With our world-leading capacity, including the Illumina, Pacbio and Oxford Nanopore platforms, we provide sequencing services for pre-made libraries at highly competitive and cost-effective prices.
Benefits of Pre-made Library Sequencing
- NGS technologies: Novogene is a top-brand in sequencing capacity using state-of-the-art technology, including Illumina HiSeq X, NovaSeq 6000 Systems, Pacbio sequel II and Oxford Nanopore promethION Beta.
- Sequencing strategy: Novogene employs PE150, PE250, PE50, SE50, and other customized set-ups.
- Extensive experience: We take pride in completing over 2000+ sequencing projects, and our data has been published in many high-profile journals.
Pre-made Library Sequencing Specifications:
Sample Requirements
| Platform | Data Amount | Volume Requirement* | Library concentration |
| HiSeq System, Paired-end 150 | Lane Sequencing | ≥ 20μL/lane (additionaL 10μL for one more lane) | ≥ 0.5 ng/μL, quantified by Qubit® 2.0 (Life Technologies)
2 nM-30 nM, quantified by |
| NovaSeq System, Paired-end 150 | < 20 G | ≥ 15 μL | |
| 20 G ≤X ≤ 100 G | ≥ 25 μL | ||
| 100 G ＜X＜400 G | ≥ 50 μL | ||
| Lane Sequencing | ≥ 50 μL/lane (additional 40μL for one more lane) | ||
| NovaSeq System, Paired-end 250, Paired-end 50, Single-end 50 | X ≤ 20 M | ≥ 15 μL | |
| 20 M＜X ≤ 50 M | ≥ 25 μL | ||
| 50 M＜ X ＜150 M* | ≥ 50 μL | ||
| Lane Sequencing | ≥ 100 μL/lane (additional 100 μL for one more lane) |
*Notes:
High concentration samples should be diluted before delivery. Pre-made libraries should be colorless.
Pre-made Library Sequencing Specifications:
Illumina Sequencing
| Platform | Read Length | Output |
| NovaSeq 6000 | PE50 | 400 M /lane 800 M /flowcell |
| SE50 | 400 M /lane 800 M / flowcell |
|
| PE250 | 400 M /lane 800 M / flowcell |
|
| PE150 | 800 G /lane 3.2 T / flowcell |
|
| HiSeq X-ten | PE150 | 110 G/lane 880 G/ flowcell |
Novogene Workflow of Pre-made Library Sequencing
For self-constructed libraries provided by the customers, quality analysis is performed initially before sequencing to ensure the quality of the data. The qualified libraries are fed into Illumina sequencers after pooling according to their effective concentrations and expected data volumes.
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