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Pre-made Library Sequencing

Introduction to Pre-made Library Sequencing

With our world-leading, powerful, and sophisticated sequencing platforms (Illumina, Pacbio, and Oxford Nanopore), we provide sequencing services for pre-made libraries at different read lengths and capacities to suit any cost, project scale, and turnaround.

Novogene accepts customers’ prepared libraries for sequencing. We have a variety of sequencing platforms that offer an assortment of sequencing read length, flux, and time cycle options. We directly sequence qualified libraries provided by customers and produce high-quality sequencing data, thus providing data basis for bioinformatics analysis by customers. Novogene has a professional and efficient operations team to ensure customer project cycle and data quality. We strive to provide customers with fast and easy sequencing services. Our services combine scientific scheme design, strict quality control management, rich project experience and high-quality project service to ensure that every link can be excellently completed to facilitate scientific research. With our world-leading capacity, including the Illumina, Pacbio and Oxford Nanopore platforms, we provide sequencing services for pre-made libraries at highly competitive and cost-effective prices.

Benefits of Pre-made Library Sequencing

  • NGS technologies: Novogene is a top-brand in sequencing capacity using state-of-the-art technology, including Illumina, NovaSeq 6000, NovaSeqX Plus systems, Pacbio sequel II/ IIe and Oxford Nanopore promethION Beta.
  • Sequencing strategy: Novogene employs PE150, PE250, PE50, SE50, and other customized set-ups.
  • Extensive experience: We take pride in completing over 2000+ sequencing projects, and our data has been published in many high-profile journals.

Pre-made Library Sequencing Specifications:
Sample Requirements

Sequencing Strategy Sequencing Platform Sequencing Data Amount Volume Requirement Library Concentration

PE150

NovaSeq 6000 S4

< 30 Gb

≥ 15 μL

≥ 2 ng/μL, quantified by Qubit® 2.0 (Life Technologies)

2 nM-30 nM, quantified by

Q-PCR

30 Gb ≤ X ≤ 100 Gb

≥ 25 μL

100 Gb < X ≤ 800 Gb

≥ 50 μL

NovaSeq 6000 S4

Lane sequencing (800G/lane)

≥ 50 μL (additional 40 μL for one more lane)

NovaSeq X Plus 10B

Lane sequencing (375G/lane)

≥ 50 μL (additional 40 μL for one more lane)

NovaSeq X Plus 25B

Lane sequencing (950G/lane)

≥ 130 μL per lane

SE50 PE50

NovaSeq 6000 SP

Flow cell sequencing

(2 lanes per flow cell)

≥ 100 μL per lane

PE250

NovaSeq 6000 SP

Lane sequencing

≥ 100 μL per lane

Pre-made Library Sequencing Specifications:
Illumina Sequencing

 

Platform Sequencing Strategy Data Output

NovaSeq X Plus 10B

PE150

375 G /lane
3.0 T / flowcell

NovaSeq X Plus 25B

PE150

950 G /lane
7.6 T / flowcell

NovaSeq 6000 S4

PE150

800 G /lane
3.2 T / flowcell

NovaSeq 6000 SP

PE50

400 M /lane
800 M /flowcell

SE50

400 M /lane
800 M / flowcell

PE250

400 M /lane
800 M / flowcell

Novogene Workflow of Pre-made Library Sequencing

For self-constructed libraries provided by the customers, quality analysis is performed initially before sequencing to ensure the quality of the data. The qualified libraries are fed into Illumina sequencers after pooling according to their effective concentrations and expected data volumes.