Shotgun metagenomic sequencing provides information on the total genomic DNA from all organisms in a sample, avoiding the need for isolation and cultivation of microorganisms or amplification of target regions. This is crucial because it is believed that nearly 99% of all microorganisms cannot be cultivated in the laboratory. Unlike the targeted approach used in the 16S/18S/ITS amplicon sequencing, shotgun metagenomic sequencing uses next-generation sequencing (NGS) technology to provide not only information on the taxonomic annotations of each organism, but also the functional profiling, gene prediction and microbial interaction of the whole community.
With extensive experience and well-developed bioinformatics pipelines, our services can help you determine the genomes, functions, and variations of microorganisms from diverse samples. Novogene’s shotgun metagenomic sequencing service delivers high-quality data, publication-ready results, and personalized analysis to meet different research objectives. Novogene offers comprehensive metagenomics solutions, from the study of community structure and species classification to system evolution, gene function, and metabolic network of environmental microorganisms.
From taxonomic profiling to functional analysis and metabolic networks, shotgun metagenomic sequencing can be used in many different research topics, including:
Note: Sample amounts displayed are for reference only. Download the Service Specifications to learn more. For detailed information, please contact us with your customized requests.
Note: Sequencing depths and analysis contents listed are for reference only. Download the Service Specifications to learn more. For detailed information, please contact us.
In order to ensure the accuracy and reliability of the sequencing data, quality control (QC), consistent with our high scientific standards, is performed at each step of the procedure. The shotgun metagenomic sequencing workflow comprises these main steps: sample preparation and quantification, fragmentation and library preparation, library quality control, sequencing, and bioinformatics analysis.
Shotgun Metagenomic Sequencing is a flexible approach to obtain microbial genomes among environments (de novo assembly), as well as gene functions (function annotation), and how they interact with each other (pathway analysis). Here presents a list of academic publications that have used Novogene Shotgun Metagenomic Sequencing services.
Effects of spaceflight on the composition and function of the human gut microbiota
Gut MicrobesIssue Date: 2020 Jul 3IF: 7.740DOI: 10.1080/19490976.2019.1710091
The chemodiversity of paddy soil dissolved organic matter correlates with microbial community at continental scales
MicrobiomeIssue Date: 19 October 2018IF: 11.607DOI: 10.1186/s40168-018-0561-x
Hypoxia induces senescence of bone marrow mesenchymal stem cells via altered gut microbiota
Nature CommunicationsIssue Date: 2018 May 22IF: 12.121DOI: 10.1038/s41467-018-04453-9
Age-associated microbiome shows the giant panda lives on hemicelluloses, not on cellulose
ISME JournalIssue Date: 01 February 2018IF: 9.180DOI: 10.1038/s41396-018-0051-y
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