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Application of Whole Transcriptome Sequencing in Cancer Research

Introduction:

Laryngeal squamous cell carcinoma (LSCC) is the second most common malignant tumor in the head and neck. Autophagy plays a critical role in cancer progression and chemoresistance. However, the function and mech-anism of circRNA in autophagy regulation of LSCC remain unclear.
Here, we present a paper titled “circPARD3 drives malignant progression and chemoresistance of laryngeal squamous cell carcinoma by inhibiting autoph-agy through the PRKCI-Akt-mTOR pathway” conducted by Wei Gao, Huina Guo, Min Niu, et al. This study integrated whole transcriptome sequencing with technical assistance from Novogene. Leveraging the advancement of circRNA sequencing, small RNA sequencing, and mRNA sequencing, the authors not only reveal the oncogenic role of circPARD3 in LSCC, but also indicate that circPARD3 can serve as a new biomarker and target for autoph-agy detection and modulation, which might be used for controlling autopha-gy levels in different developmental stages of LSCC.

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