Despite the fact that several large-scale RNA-seq studies have been performed in plants to analyse AS, currently it is not known how many distinct splice isoforms are produced. Here, in a paper titled ‘‘A survey of the sorghum transcriptome using single-molecule long reads’’ from Program in Cell and Molecular Biology, Colorado State University, the researchers integrated PacBio Isoform Sequencing (Iso-Seq) to transcriptome analysis on sorghum transcriptome, to identify full-length splice isoforms and alternative polyadenylation (APA) sites. This Paper provided the first comprehensive view of splice variants in sorghum, underscoring the advantage of Iso-Seq in identifying full-length splice isoforms. For more details, please download our case study right now！
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