A Beginner’s Guide to Microbial Sequencing – 16S/18S/ITS Amplicon Sequencing vs. Shotgun Metagenomic Sequencing
Shotgun metagenomic sequencing provides information on the total genomic DNA from all organisms in a sample, avoiding the need for isolation and cultivation of microorganisms or amplification of target regions. This is crucial because it is believed that nearly 99% of all microorganisms cannot be cultivated in the laboratory. Differently from the targeted approach used in the 16S/18S/ITS amplicon sequencing, shotgun metagenomic sequencing uses next-generation sequencing (NGS) technology to provide not only information on the taxonomic annotations of each organism but also the functional profiling, gene prediction and microbial interaction of the whole community.
- A general analysis of both 16S/18S/ITS amplicon metagenomic sequencing and shotgun metagenomic sequencing
- Gain insights into a variety of research strategies