The opportunity to provide researchers with an unprecedented insight into the transcriptional landscape of cells by using RNA sequencing (RNA-seq) has boosted many important discoveries. However, RNA-seq is generally performed in bulk, which measures average gene expression levels based on thousands to millions of cells. Benefitting from cutting-edge Next GEM technology powered by 10x Genomic in recent years, single-cell RNA-seq makes it possible to partition each single cell from a pool of cells and label each single cell, simultaneously. In turn, it can reveal cell-to-cell differences and cellular heterogeneities that are often obscured by bulk RNA-seq. Until now, 10x single-cell RNA-seq has been successfully applied to manifold research fields.

Learning Objectives:

• Walk you through the world of single-cell RNA sequencing. From sample submission to library preparation to sequencing and bioinformatic analysis pipeline
• A better understanding of how 10x single-cell RNA sequencing is applied to the liver disease research