Sample Preparation Manual for Single Cell Sequencing
Introduction:
This manual provides comprehensive guidelines for the preparation of samples for single cell sequencing. Precision in sample handling and preparation is crucial to ensure data quality and integrity. The protocols outlined herein are optimized to preserve cell viability, minimize technical variation, and maximize the success of downstream applications. Whether working with fresh, frozen, or dissociated tissue samples, users will find best practices and recommendations for every critical step of the workflow.