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Isoform Sequencing FAQ: The Latest News You Need to Know on Iso- Seq

Introduction:

Isoform sequencing (Iso-Seq) is a long-read sequencing technique developed by PacBio that uses Single-Molecule Real-Time (SMRT) technology to capture full-length transcript isoforms without the need for transcript assembly. By utilizing Circular Consensus Sequencing (CCS) and high-quality long reads (HiFi reads), Iso-Seq provides high-resolution insights into transcriptome complexity, enabling the discovery of novel isoforms, alternative splicing events, and precise annotation of genome.

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