DNA sequencing development revolutionized biological and medical sciences 50 years ago. Next-generation sequencing is the modern, second revolution and the spearhead of an ever-accelerating field. Here is what you need to know to begin using this tool.
The history of genetic research is rather new. From the presentation of Mendel’s discoveries in the 1860s to the development of the Central Dogma of molecular biology in the 1950s, we came a long way in understanding genetics. However, we still couldn’t tap into the information contained inside the DNA. It was only in the 1970s that the science of genetics as we know it truly started to thrive with the development of Sanger’s chain-termination method, the first-generation sequencing. After the first automated sequencing machine development in 1987, we witnessed a fast-paced and dynamic expansion in genetics and genomics over the next 20 years.
The development of the sequencing by synthesis (SBS) method in the 2000s marked the start of a new era. This method, also known as second-generation sequencing or next-generation sequencing (NGS), allows the massively parallel sequencing of millions of DNA fragments simultaneously. Essentially, sequencing costs[a] dropped by an order of 106 and the sequencing speed increased by up to an order of 106. It is now feasible to confidently and cost-efficiently tackle research avenues that require whole genome sequencing (WGS), resequencing and de novo genome sequencing, transcriptome, and epigenetics studies, of both eukaryotic and prokaryotic organisms.
The workflow for a typical Illumina NGS has four steps.
Novogene can help you with a range of NGS applications. Please, contact your regional sales manager to explore more.
We are proud to be able to support your research by generating high-quality, publication-ready data in a rapid time-frame. Reach out to us and we will get back to you shortly.
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