Introduction to Pre-made Library Sequencing

We offer tailored sequencing services using cutting-edge sequencing platforms, including Illumina, PacBio, and Oxford Nanopore. Our comprehensive solutions accommodate libraries prepared by customers, providing various read lengths and capacities to meet diverse project needs. Whether you require a cost-effective option, a large-scale endeavor, or a rapid turnaround, our services can align with your specific requirements.
Novogene offers sequencing services for customer-prepared, ready-to-sequence libraries. Our diverse portfolio of sequencing platforms provides flexibility in read lengths, throughput, and turnaround times. We sequence various compatible library types provided by customers, delivering high-quality sequencing data to serve as a foundation for bioinformatics analysis.
Our experienced team are committed to providing customers efficient and seamless sequencing services, integrating scientific expertise, rigorous quality control, extensive project experience and efficient operations to ensure excellence at every stage, facilitating scientific research.
Why Choose Novogene?
- State-of-the-art NGS Capacity: Novogene is a top-brand in sequencing capacity using state-of-the-art technology, including Illumina NovaSeqX Plus systems, NovaSeq 6000, Pacbio Revio, sequel II/ IIe and Oxford Nanopore promethION Beta.
- Flexible sequencing strategy: Novogene employs PE150, PE250, PE50, SE50, and other customized sequencing modes.
- Trusted Expertise: Rely on the expertise of Novogene’s experienced team to guide you through every step of your sample processing.
- Rapid Turnaround Time: Novogene offers the fastest turnaround time upfront so you can benefit from quicker sample processing from QC to data release, allowing you to accelerate your project timelines.
Pre-made Library Sequencing Specifications:
Sample Requirements
Sequencing Strategy | Sequencing Platform | Sequencing Data Amount | Volume Requirement | Library Concentration |
PE150 |
NovaSeq X Plus Partial |
50G or 100G |
≥ 30 μL |
≥ 2 ng/μL, quantified by Qubit® 2.0 (Life Technologies) 2 nM-30 nM, quantified by Q-PCR |
100 G ≤ X ≤ 400 G |
≥ 50 μL |
|||
400 G < X ≤ 1000 G |
≥ 130 μL |
|||
NovaSeq 6000 S4 |
Lane sequencing (800G/lane) |
≥ 50 μL (additional 40 μL for one more lane) |
||
NovaSeq X Plus 10B |
Lane sequencing (375G/lane) |
≥ 50 μL (additional 40 μL for one more lane) |
||
NovaSeq X Plus 25B |
Lane sequencing (1000G/lane) |
≥ 130 μL per lane |
||
SE50 PE50 |
NovaSeq 6000 SP |
Flow cell sequencing |
≥ 200 μL per flow cell |
|
PE250 |
NovaSeq 6000 SP |
Flow cell sequencing |
≥ 200 μL per flow cell |
Pre-made Library Sequencing Specifications:
Illumina Sequencing
Platform | Sequencing Strategy | Data Output |
NovaSeq X Plus 10B |
PE150 |
375 G /lane |
NovaSeq X Plus 25B |
PE150 |
1000 G /lane |
NovaSeq 6000 S4 |
PE150 |
800 G /lane |
NovaSeq 6000 SP |
PE50 |
400 M /lane |
SE50 |
400 M /lane |
|
PE250 |
400 M /lane |
Novogene Workflow of Pre-made Library Sequencing
More Research Services
