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De novo Sequencing

De novo sequencing is a powerful approach that constructs the complete genomic sequence of an organism without relying on an existing reference genome. This method is particularly valuable for studying species with no prior genomic data, enabling researchers to explore uncharted genetic landscapes. Services can be applied to research of animals, plants, and microorganisms, including phylogenetic studies, analysis of species diversity, genetic markers, and other genomic research.

Applications of De Novo Sequencing

De novo sequencing is widely used across diverse fields, including:

  • Animal Genomics: Study genetic diversity, evolutionary relationships, and species-specific traits.
  • Plant Genomics: Accelerate crop improvement, enhance disease resistance, and uncover novel genetic markers.
  • Microbial Genomics: Investigate microbial diversity, identify new species, and analyze functional gene clusters.

Technology

Sequencing Platform

  • ILLUMINA

    NovaSeq 6000

    System specifications:

    80Gb - 6Tb output range, 1.6 - 40 B paired end reads per run, and PE150, PE250, PE50, etc.

  • ILLUMINA

    HiSeq X

    System specifications:

    1.6 – 1.8 Tb output range, 5.3 – 6 B reads per run, and 2 x150 bp max read length.

  • PACBIO

    PacBio Revio System

    System specifications:

    N50>15kb, 90% of bases ≥Q30, 90Gb HiFi yield / SMRT Cell, 360Gb HiFi yield / run, 24-hour run times etc.

  • PacBio

    PacBio Sequel IIe System

    System specifications:

    N50>15kb, read lengths up to 25kb (CCS), >99.999% (QV50) consensus accuracy, coverage across high-GC/repeat regions.

  • Nanopore

    Nanopore PromethION

    System specifications:

    Ultra-long reads up to 2Mb, high yields for large genomes, REAL real-time and accessible for flexible budgets.