Pre-made Library Sequencing on Illumina Platforms
Novogene offers sequencing-only services for customer-prepared, ready-to-sequence libraries on the state-of-the-art NovaSeq X Plus platform and our diverse portfolio of sequencing solutions provides flexibility in read lengths, throughput, and turnaround times to meet various demands. We launched a new sequencing solution, Pre-made Library Swift Sequencing service, designed to deliver data as fast as 5 days and to propel customers’ research.
Our experienced team are committed to providing customers efficient and seamless sequencing services, integrating scientific expertise, rigorous quality control, extensive project experience and efficient operations to ensure excellence at every stage, facilitating scientific research.
Why Choose Illumina Seq-Only Services with Novogene?
- Cost-Efficiency: Take advantage of the lowest sequencing costs, on a per read/Gb basis, with a guaranteed Q30 score ≥ 85%.
- Trusted Expertise: Trust Novogene’s experienced team to guide you through every step of your sample processing, ensuring reliable and high-quality results.
- Rapid Turnaround Time: Take advantage of our fastest turnaround times, included at no extra cost, to benefit from quicker sample processing from QC to data release, helping you meet your project deadlines efficiently.
- Flexible Project Options: Take control of your sequencing projects with our flexible options, including multiple lane/ data amount purchases, compatibility across a diverse range of library types and customized sequencing parameters.
- US-Based Processing:Two US lab locations (Davis Lab and Beaverton Lab) for processing and sequencing.
- Automated Workflow:Novogene’s Customer Service System (CSS) to streamline project management integrated with all-in-one cloud bioinformatics platform, NovoMagic, for efficient data analysis and visualization.
Flexible Partial Lane Sequencing on NovaSeq X Plus
Our Partial Lane Sequencing service allows multiple projects to share a lane on the NovaSeq X Plus, offering economic flexibility and easy access to small data volume options using the latest sequencing technology.
We welcome all Illumina-compatible libraries that meet our requirements for Partial Lane Sequencing. These libraries are expertly grouped and balanced with other samples to ensure optimal base diversity on the lane, eliminating the need for PhiX spike-in libraries as balancers and maximizing usable lane space.
Reliable Full Lane Sequencing on NovaSeq X Plus
Our Full Lane Sequencing service offers entire lanes of the flow cell on NovaSeq X Plus for exceptional throughput and sequencing consistency. By minimizing cross-contamination risks, the Full Lane Sequencing ensures reliability and precision of your sequencing results.
To maintain high-quality data from NovaSeq X Plus, it’s essential to preserve base diversity on the lane. We recommend selecting index adapters with diverse index sequences that optimize color balance within pooled libraries. This strategy is essential for successful demultiplexing and subsequent data analysis. As a general guideline, Illumina recommends selecting index sequences such that signal will be present in both channels for each index cycle when sequencing libraries with two-color chemistry.
Project Deliverables
- Raw Data in FASTQ files with Quality Control Report
- Flexible Bioinformatics Analysis (upon request)
Pre-made Library Sequencing Specifications:
Sample Requirements
| Sequencing Strategy | Sequencing Platform | Sequencing Data Amount | Volume Requirement | Library Concentration |
|
PE150 |
NovaSeq X Plus Partial |
50G or 100G |
≥ 30 μL |
≥ 2 ng/μL, quantified by Qubit® 2.0 (Life Technologies) 2 nM-30 nM, quantified by Q-PCR |
|
100 G ≤ X ≤ 400 G |
≥ 50 μL |
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|
400 G < X ≤ 1000 G |
≥ 130 μL |
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|
NovaSeq 6000 S4 |
Lane sequencing (800G/lane) |
≥ 50 μL (additional 40 μL for one more lane) |
||
|
NovaSeq X Plus 10B |
Lane sequencing (375G/lane) |
≥ 50 μL (additional 40 μL for one more lane) |
||
|
NovaSeq X Plus 25B |
Lane sequencing (1000G/lane) |
≥ 130 μL per lane |
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|
SE50 PE50 |
NovaSeq 6000 SP |
Flow cell sequencing |
≥ 200 μL per flow cell |
|
|
PE250 |
NovaSeq 6000 SP |
Flow cell sequencing |
≥ 200 μL per flow cell |
Novogene Workflow of Pre-made Library Sequencing
For self-constructed libraries provided by the customers, quality analysis is performed initially before sequencing to ensure the quality of the data. The qualified libraries are fed into Illumina sequencers after pooling according to their effective concentrations and expected data volumes.
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