ATAC-seq is a high-throughput sequencing method for analyzing genome-wide chromatin accessibility, which is essential for understanding the global epigenetic control of gene expression. Chromatin accessibility refers to the degree to which nuclear macromolecules can physically interact with chromatinized DNA, make it possible to transcript and process other biological progress. Therefore, ATAC-seq provides insights into the genome’s regulatory landscape by revealing regions where DNA is accessible for binding by various cellular factors.
Based on Next Generation Sequencing(NGS) technology with illumina NovaSeq platform, Novogene’s ATAC-seq service empowers researchers to explore genome-wide chromatin accessibility, a critical factor in gene expression regulation. During the library preparation of ATAC-seq, sequencing adapters are inserted into open chromatin regions using the hyperactive Tn5 transposase. All open chromatin regions can be captured under a specific space-time condition by ATAC-seq for sequencing, without being limited to the binding sites of a transcription factor or a certain histone-modified region.
The Novogene ATAC-seq service comprises four steps: Sample preparation, library preparation, sequencing, and bioinformatics analysis. The workflow cannot be paused after sample thawing due to the nature of the initial samples (cells/tissues). Please contact us for more information about your ATAC-seq projects. To guarantee the accuracy and reliability of sequencing data, Novogene audits every experimental step through quality control, fundamentally ensuring high-quality data output from sampling to the final data report. This commitment to high-quality data is essential for the correctness, comprehensiveness, and credibility of bioinformatics analysis.
Motif indicates the sequence conservation of the position of the peak, which may play a role in the regulation of gene expression.
Note: The most significant 25 motifs of lengths 8, 10, 12, 14 were identified for each set of experiments. More detailed information for each motif is available in the result file and the readme file.
Each gene, along with its 3kb upstream and 3kb downstream regions, is divided into bins according to the window size of 50bp. Full reports include additional analysis results related to the TSS (Transcription Start Site) following peak calling.
The distribution of peaks across various functional areas is shown below. The correspondence between peaks and functional areas follows the priority order of promoter, 5’UTR, 3’UTR, Exon, Intron, Downstream, and Intergenic. Additional enrichment analysis is also included in full reports.
GO and KEGG enrichment analysis annotate differential peaks between groups enriched in the biological process, cellular component, molecular function, and biological pathway.
Note: Differential analysis can be performed between groups only when there are two or more groups.
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