Genome Sequencing
Novogene’s genome sequencing services help to meet a wide range of research goals. Our genome sequencing services encompass projects that require resequencing to compare samples against a reference genome as well as projects that require de novo sequencing to generate reference quality genomes of seldom-sequenced species.
Whole Genome Sequencing (WGS), focused on resequencing, can be utilized in a wide range of research studies including evolutionary biology, developmental biology, agriculture, microbiology, and biomedical research.
Our de novo Whole Genome sequencing service provides genome assembly and annotation for species, that have either never been sequenced before or species with missing or incomplete reference genomes.
Targeted Resequencing services can be a cost-effective alternative for in-depth analysis of a select region of the genome, such as WES (Whole Exome Sequencing) for studying exomes (protein-coding regions of the genome) or TRS (Targeted Region Sequencing) for investigating a select set of genes or genomic regions.
Technology
Sequencing Platforms
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ILLUMINA
NovaSeq X Plus
System specifications:Up to 16 Tb of output (or up to 52 billion single reads) per dual 25B flow cell run. Run time of nearly 48 h. Q30 ≥85% (PE150)
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ILLUMINA
NovaSeq 6000
System specifications:80Gb - 6Tb output range, 1.6 - 40 B paired end reads per run, and PE150, PE250, PE50, etc.
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PACBIO
PacBio Revio System
System specifications:N50>15kb, 90% of bases ≥Q30, 90Gb HiFi yield / SMRT Cell, 360Gb HiFi yield / run, 24-hour run times etc.
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PacBio
PacBio Sequel II/IIe System
System specifications:N50>15kb, read lengths up to 25kb (CCS), >99.999% (QV50) consensus accuracy, coverage across high-GC/repeat regions. HiFi reads for PacBio Sequel IIe only.
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Nanopore
Nanopore PromethION
System specifications:Ultra-long reads up to 2Mb, high yields for large genomes, REAL real-time and accessible for flexible budgets.
