Introducing PlasmidGo - ONT-based Whole Plasmid Sequencing by Novogene Europe
As your trusted one-stop sequencing service provider, Novogene Europe proudly presents PlasmidGo, our new Oxford Nanopore Technologies (ONT)-based Whole Plasmid Sequencing service.
Plasmids are the essential companions of every molecular biology lab. With PlasmidGo, you can streamline your cloning workflow through fast, accurate plasmid sequencing with a 1-day turnaround. Powered by ONT’s long-read sequencing platform, PlasmidGo delivers complete, high-accuracy plasmid maps, helping your plasmid projects go further with confidence and ease.
Why Choose ONT-based Whole Plasmid Sequencing?
As a superior alternative to traditional Sanger sequencing, ONT-based Whole Plasmid Sequencing provides a comprehensive, uninterrupted view of your plasmid construct. Using long-read technology, this approach accurately resolves complex variants and repetitive regions often missed by other methods.
Raw reads generated with ONT’s latest Q20+ chemistry offer high coverage depth, and the resulting consensus sequences are processed into a high-quality, fully assembled plasmid map.
Key Advantages Over First-Generation Plasmid Sequencing
- Effortlessly spans fragments of tens of kilobases
- Provides single-molecule resolution for superior accuracy
- Captures a complete plasmid picture, including challenging or repetitive regions
ONT-based Whole Plasmid Sequencing Specifications - Sample Requirement
| Sample Type | Amount | Volume | Concentration (Qubit) | Purity | Dissolution Buffer |
| Plasmid DNA (2~25Kb) | ≥ 400ng | ≥ 10 μL | ≥ 10ng/μL, with distinct bands visible by gel electrophoresis | OD260/280 = 1.8-2.0, OD260/230 = 2.0-2.2; free of contaminants (detergents, salts, proteins) | ultrapure water (double-distilled water/Milli-Q) or Elution Buffer without EDTA |
Novogene Workflow of Whole Plasmid Sequencing
We deliver a streamlined end-to-end workflow for whole plasmid sequencing
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SlyB encapsulates outer membrane proteins in stress-induced lipid nanodomains
NATUREIssue Date: 11 December 2023IF: 64.8DOI: 10.1038/s41586-023-06925-5
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Profiling of the Helicobacter pylori redox switch HP1021 regulon using a multi-omics approach
Nature CommunicationsIssue Date: 23 October 2023IF: 16.6DOI: 10.1038/s41467-023-42364-6
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PLANT CELLIssue Date: 24 November 2023IF: 11.6DOI: 10.1093/plcell/koad292
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ISME JournalIssue Date: 10 January 2024IF: 11DOI: 10.1093/ismejo/wrad001
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Plasmids manipulate bacterial behaviour through translational regulatory crosstalk
PLOS BIOLOGYIssue Date: 14 February 2023IF: 9.593DOI: 10.1371/journal.pbio.3001988
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A Deep-Sea Bacterium Is Capable of Degrading Polyurethane
Microbiology SpectrumIssue Date: 30 March 2023IF: 9.043DOI: 10.1128/spectrum.00073-23
1.Plasmid Assembly and Statistics
2.Consensus Sequence and Annotation
3.Quality Control and Visualisation
3.1 Read length histogram
3.2 coverage plot
3.2 Chromatogram
4.Per-base data (variant file)
