Single Cell RNA Sequencing 

Single-cell RNA sequencing (scRNA-seq) takes transcriptomics analysis a step further, providing unprecedented insights into the individual cellular heterogeneity within a population of seemingly identical cells in complex biological systems. This modern transcriptomics technology builds upon the foundations of bulk RNA-seq by incorporating microfluidics or other single-cell isolation techniques to keep track of RNA from individual cells. Generally speaking, scRNA-seq captures the mRNA from individual cells, converts it into complementary DNA (cDNA), and constructs barcoded libraries for high-throughput sequencing. By examining gene expression at the cellular level, scRNA-seq enables the identification of rare cell types, elucidates cellular states, enhances the understanding of disease progression and mechanisms, and identifies developmental trajectories, among others. 

Importantly, scRNA-seq has become a powerful tool for immune profiling at the single-cell level, allowing detailed characterization of immune cell populations, activation states, and functional diversity, as well as the analysis of immune receptor repertoires and cell–cell interactions. These capabilities significantly enhance the understanding of immune responses in health and disease, including cancer, infection, autoimmunity, and inflammatory disorders. 

Empower your single cell omics research with Novogene’s end-to-end workflow powered by 10X Genomics’ GEM-X technology.