Introduction to lncRNA Sequencing
Long non-coding RNAs (lncRNAs) are a moderately abundant fraction of the eukaryotic transcriptome, which are comprised of longer than 200nt non-coding RNAs (ncRNAs) , including lincRNAs (intergenic lncRNAs), intronic, anti-sense, sense and bidirectional lncRNAs, which do not encode proteins. lncRNAs affect multiple cellular functions through their regulation of gene transcription, post-transcriptional modifications, and epigenetics. lncRNA sequencing (lncRNA-seq) is a powerful NGS tool to study functional roles in diverse biological processes and human diseases, such as cancer and neurological disorders.
The progressive library preparation of Novogene’s lncRNA-seq service enables information enrichment and gene expression profiling for both coding and non-coding transcripts from a highly sensitive transcriptomic perspective. Through the application of bioinformatics analysis, the strand orientation and the regulatory association between lncRNA and targeted mRNA can be investigated, all in a single run.
Applications of lncRNA sequencing
LncRNA-seq has been opening new and exciting research possibilities, including:
- Profiling known and novel transcripts and identifies variations
- Predicting target genes of lncRNA
- Identifying biomarkers for cancer/disease diagnostics and classification
- Discovering regulation between lncRNA and mRNA
Benefits of Novogene lncRNA sequencing
- Novogene lncRNA-seq offers high throughput and high accuracy (with Q30 score ≥ 85%) coupled to low initial RNA amounts required. Novogene has extensive experience providing RNA-Seq services, having successfully completed thousands of projects and helped multiple researchers to publish in high Impact Factor journals.
- Novogene offers inclusive solutions for lncRNA identification, quantification, differential expression, enrichment analysis, new gene prediction and target prediction. Highly-qualified bioinformaticians deliver publication-ready analysis results using personalized pipelines.
- Quick shipment to our UK or German lab.
Eukaryotic ncRNA Sequencing Service Flyer
Eukaryotic ncRNA-seq uses a next-generation sequencing approach to reveal the profile of non-coding RNAs at certain timepoints or under specific conditions. Sequencing data allows for the identification of present transcript isoforms and their abundance. Novogene’s Eukaryotic ncRNA-seq service delivers high quality data, publication-ready analysis results, and personalized analysis pipelines for long noncoding RNA, circular RNA, and small RNA. With our whole transcriptome sequencing service, the potential transcriptional and regulatory networks among different types of RNAs are studied.
On-demand Webinar
ncRNA-seq Results Explained: What you can explore with non-coding RNA data
Non-coding RNA-sequencing (ncRNA-seq) is a powerful technique for studying the regulatory functions of different types of ncRNAs in gene expression: from long non-coding RNA sequencing (lncRNA-seq), small RNA sequencing (sRNA-seq) to circular RNA sequencing (circRNA-seq) for diverse eukaryotic species.
The data generated by ncRNA-seq can uncover the gene expression profile of coding and non-coding transcripts and assist in the discovery of novel transcripts, to establish regulation networks among lncRNA, miRNA and circRNA.
However, without a background in bioinformatics, the analysis required can be both challenging and intimidating. In this follow up webinar to our ‘RNA-seq Results Explained’, we provide an overview of the ncRNA-seq analysis workflow, and a walkthrough of the tools and processes used to assess your datasets and meet your research goals.
Learning Objectives:
- Get an overview of ncRNA sequencing approaches
- Learn about ncRNA-seq data evaluation & visualisation
- Get familiar with data handling software and file formats.
lncRNA-seq Specifications: RNA Sample Requirements
| Library Type | Sample Type | Amount | RNA Integrity Number (Agilent 2100) | Purity (NanoDrop) |
| lncRNA Library | Total RNA | ≥ 500 ng | Animal ≥ 6.5, Plant ≥ 6, with smooth baseline |
A260/280 = 1.8-2.2 A260/230 ≥ 1.8 |
| Exosomal lncRNA Library | Exosomal RNA | ≥ 5 ng | Peak between 25-200 nt, FU> 10, no peak > 2000 nt |
|
| Dual RNA library | Total RNA | ≥ 1 μg | ≥ 6.5, with flat base line |
Note: Sample amounts displayed are for reference only. Download the Service Specifications or Sample Requirements to learn more. For detailed information, please contact us.
lncRNA-seq Specifications: Sequencing and Analysis
| Sequencing Platform | Illumina NovaSeq X Plus |
| Recommended Data Output | ≥ 40 million read pairs per sample |
| Recommended Sequencing Depth | ≥ 40 million read pairs per sample for species with reference genome |
| Standard Data Analysis |
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Note: Parameters are displayed for reference only. Download the Service Specifications to learn more. For detailed information, please contact us.
Novogene lncRNA Sequencing Project Workflow
The workflow of the lncRNA-seq starts with sample preparation and quality control. The ribosomal RNA (rRNA) is depleted for target transcript enrichment. The fragmented RNA undergoes reverse transcription into cDNA. Strand-specific libraries (also known as stranded libraries, directional libraries) are prepared, and the sequencing is performed using a paired-end 150bp strategy on the Illumina platform. The downstream processing follows the well-established Novogene pipeline, which guarantees the highest quality results. Customized bioinformatics solutions are available upon request.
Featured publications using Novogene lncRNA service
lncRNA-seq is a powerful tool that not only reveals the quantification and functional enrichment of the target transcripts but also indicates the strand orientation and regulatory relationship between lncRNA and targeted mRNA. There list some exciting publications that have used Novogene’s lncRNA-seq services.
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Absence of Scaffold Protein Tks4 Disrupts Several Signaling Pathways in Colon Cancer Cells
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCESIssue Date: 9 January 2023IF: 6.208DOI: 10.3390/ijms24021310
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BiomoleculesIssue Date: 5 April 2024IF: 5.5DOI: 10.3390/biom14040445
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Celll ReportsIssue Date: 2020IF: 8.109DOI: 10.1016/j.celrep.2020.107694
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Small Protein Hidden in lncRNA LOC90024 Promotes “Cancerous” RNA Splicing and Tumorigenesis
Advanced ScienceIssue Date: 11 March 2020IF: 15.840DOI: 10.1002/advs.201903233
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Molecular PsychiatryIssue Date: 03 February 2020IF: 12.384DOI: 10.1038/s41380-020-0662-3
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Plant Biotechnology JournalIssue Date: 16 August 2019IF: 8.154DOI: 10.1111/pbi.13234
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rSj16 Protects against DSS-Induced Colitis by Inhibiting the PPAR-α Signaling Pathway
TheranosticsIssue Date: 2017IF: 8.579DOI: 10.7150/thno.20359
Alternative Splicing
lncRNA Identification
Group Comparison
Volcano Plot of Transcript Quantification
Clustering of mRNA
Clustering of lncRNA
Clustering of TUCP
