Metagenome Sequencing
Metagenomics is the study of microbial communities in their original habitats and gives a comprehensive insight into the biochemical and metabolic interactions within these communities. Metagenomics can also help identify individual species within microbial habitats with no pre-isolation required. Metagenomics is also used to compare differentially expressed genes within various functional pathways across alternative environments. It reveals the adaptive mechanisms of microorganisms under different environmental stress and explores the interactions between them and other components of their surroundings.
Technology
Sequencing Platforms
-
ILLUMINA
NovaSeq X Plus
System specifications:Up to 16 Tb of output (or up to 52 billion single reads) per dual 25B flow cell run. Run time of nearly 48 h. Q30 ≥85% (PE150)
-
ILLUMINA
NovaSeq 6000
System specifications:80Gb - 6Tb output range, 1.6 - 40 B paired end reads per run, and PE150, PE250, PE50, etc.
-
PACBIO
PacBio Revio System
System specifications:N50>15kb, 90% of bases ≥Q30, 90Gb HiFi yield / SMRT Cell, 360Gb HiFi yield / run, 24-hour run times etc.
-
PacBio
PacBio Sequel II/IIe System
System specifications:N50>15kb, read lengths up to 25kb (CCS), >99.999% (QV50) consensus accuracy, coverage across high-GC/repeat regions. HiFi reads for PacBio Sequel IIe only.
-
Nanopore
Nanopore PromethION
System specifications:Ultra-long reads up to 2Mb, high yields for large genomes, REAL real-time and accessible for flexible budgets.
