De novo Sequencing
De novo whole genome sequencing is a method to sequence the genome of an organism, without relying on a reference genome. This approach is particularly useful for studying organisms with no previously sequenced genome or for identifying novel genes and genetic variations, that might be missed when using a reference-based approach. At Novogene, we offer both short read and long-read sequencing platforms to help you achieve de-novo assemblies of reference quality genome. De novo sequencing approach can be applied to animals, plants, and microorganisms, to study diverse fields including evolutionary biology, developmental biology, mutagenesis studies, agriculture, biomedical sciences and others.
If you are interested in applications requiring resequencing instead, please refer here.
Technology
Sequencing Platforms
-
ILLUMINA
NovaSeq X Plus
System specifications:Up to 16 Tb of output (or up to 52 billion single reads) per dual 25B flow cell run. Run time of nearly 48 h. Q30 ≥85% (PE150)
-
ILLUMINA
NovaSeq 6000
System specifications:80Gb - 6Tb output range, 1.6 - 40 B paired end reads per run, and PE150, PE250, PE50, etc.
-
PACBIO
PacBio Revio System
System specifications:N50>15kb, 90% of bases ≥Q30, 90Gb HiFi yield / SMRT Cell, 360Gb HiFi yield / run, 24-hour run times etc.
-
PacBio
PacBio Sequel II/IIe System
System specifications:N50>15kb, read lengths up to 25kb (CCS), >99.999% (QV50) consensus accuracy, coverage across high-GC/repeat regions. HiFi reads for PacBio Sequel IIe only.
-
Nanopore
Nanopore PromethION
System specifications:Ultra-long reads up to 2Mb, high yields for large genomes, REAL real-time and accessible for flexible budgets.
