Targeted Resequencing
Targeted Resequencing allows researchers to extract sequence information from a specific region of interest. This could be either from protein coding regions of the genome (exome) using WES (Whole exome sequencing) or from a subset of genes or genomic region using TRS (Target Region sequencing).
Novogene’s Targeted Resequencing services, including Whole Exome Sequencing (WES) and Target Region Sequencing (TRS) are suitable for researchers interested in specific genomic regions, rather than the genome in entirety. Targeted Resequencing is a cost-effective alternative to whole genome sequencing by delivering higher coverage for regions of interest and aids in the identification of low-frequency variants.
Technology
Sequencing Platforms
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ILLUMINA
NovaSeq X Plus
System specifications:Up to 16 Tb of output (or up to 52 billion single reads) per dual 25B flow cell run. Run time of nearly 48 h. Q30 ≥85% (PE150)
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ILLUMINA
NovaSeq 6000
System specifications:80Gb - 6Tb output range, 1.6 - 40 B paired end reads per run, and PE150, PE250, PE50, etc.
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PACBIO
PacBio Revio System
System specifications:N50>15kb, 90% of bases ≥Q30, 90Gb HiFi yield / SMRT Cell, 360Gb HiFi yield / run, 24-hour run times etc.
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PacBio
PacBio Sequel II/IIe System
System specifications:N50>15kb, read lengths up to 25kb (CCS), >99.999% (QV50) consensus accuracy, coverage across high-GC/repeat regions. HiFi reads for PacBio Sequel IIe only.
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Nanopore
Nanopore PromethION
System specifications:Ultra-long reads up to 2Mb, high yields for large genomes, REAL real-time and accessible for flexible budgets.
