Introduction to scRNA-seq
Single-cell RNA sequencing (scRNA-seq) takes transcriptomics analysis a step further, providing unprecedented insights into the individual cellular heterogeneity within a population of seemingly identical cells in complex biological systems. This modern transcriptomics technology builds upon the foundations of bulk RNA-seq by incorporating microfluidics or other single-cell isolation techniques to keep track of RNA from individual cells. Generally speaking, scRNA-seq captures the mRNA from individual cells, converts it into complementary DNA (cDNA), and constructs barcoded libraries for high-throughput sequencing. By examining gene expression at the cellular level, scRNA-seq enables the identification of rare cell types, elucidates cellular states, and enhances the understanding of disease progression and mechanisms, and developmental biology, among others.
At Novogene, our single-cell sequencing solutions are powered by the latest 10X Genomics’ Chromium iX platform and the newest GEM-X chemistry, providing researchers with hassle-free, accurate and reliable results for their research. Our standardized, efficient end-to-end workflow is complete with sample processing and QC, single-cell library preparation, sequencing on the NovaSeq X Plus and bioinformatics analysis to assist with data interpretation, including the generation of “publication-ready’’ figures for easy data visualization.
scRNA-seq has a broad range of applications in both academic research and in biopharmaceutical industry, including supporting studies requiring single-cell resolution for understanding gene expression heterogeneity, identification of novel and rare cell types, biomarker discovery and beyond.
Applications of scRNA-seq
scRNA-seq has been assisting researchers in the following research areas, including:
- Developmental biology
- Cancer research
- Neuroscience
- Immunology
Benefits of Novogene’s scRNA-seq
- End-to-end service: Our seamless end-to-end scRNA-seq service includes sample processing & QC, single-cell library preparation, sequencing on the NovaSeq X Plus and even bioinformatics analysis with “publication-ready’’ figures.
- Extensive Experience: Novogene has extensive global experience with offering scRNA-seq sequencing for a large variety of species and tissue types.
- High-Quality Data: We offer data output guarantee and high-quality sequencing data that meets leading industry standards, for samples that pass our quality control standards.
- Bioinformatics Support: We provide comprehensive bioinformatics support, including 10X Genomics’ Cell Ranger analysis, to help researchers gain deeper insights from their biological data. Whether you need standard pipelines utilizing latest analysis algorithms in the field or tailored solutions, we’ve got you covered.
- Latest technology: Leveraging 10x Genomics’ latest Chromium iX and the newest GEM-X chemistry, we provide state-of-the-art scRNA-seq services.
- Fast Turnaround Times: Our scRNA-sequencing lab is located near you, in Munich, Germany, allowing for reduced shipping times. With our large sequencing capacity, automated and streamlined workflows, we ensure that you receive your data promptly without compromising on quality.
- Customer Service System (CSS): Novogene’s Customer Self Service System (CSS) is an online portal that streamlines project management and provides direct access to all your sequencing projects in one place. You can view your sequencing project(s), track their progress, access reports, collaborate with your team members and obtain other useful information in one place.
scRNA-seq specifications: Sample requirements
| Service | Sample Type | Amount | Remark |
| scRNA-seq | Frozen tissues | 50-200mg | Needs to be shipped with dry ice |
scRNA-seq specifications: Sequencing and analysis
| Sequencing Platform | Illumina NovaSeq X Plus Sequencing System |
| Read length | Paired-end 150 bp (Trimmed data is delivered) |
| Data Output | Recommended: 100-150 Gb per sample* *Number of reads/cell can be increased depending on the research application |
| Data Analysis Capability |
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Project workflow of Novogene scRNA-seq service
