{"id":36280,"date":"2023-07-19T03:17:35","date_gmt":"2023-07-19T10:17:35","guid":{"rendered":"https:\/\/www.novogene.com\/us-en\/?post_type=resources&p=36280"},"modified":"2023-10-22T23:46:25","modified_gmt":"2023-10-23T06:46:25","slug":"advancing-polyploid-assembly-and-large-genome-assembly-in-plants-through-long-read-sequencing","status":"publish","type":"resources","link":"https:\/\/www.novogene.com\/us-en\/resources\/publications\/advancing-polyploid-assembly-and-large-genome-assembly-in-plants-through-long-read-sequencing\/","title":{"rendered":"Advancing Polyploid Assembly and Large-Genome Assembly in Plants Through Long-Read Sequencing"},"content":{"rendered":"
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Introduction:<\/h5>\n

High-quality reference genomes are complete, contiguous, accurate, and representative of a given species. They are highly valued in scientific research because they serve as the foundation for studying gene function, gene expression, organismal evolution, genetic variation, disease-causing mutations, epigenomics, and for comparative genomics across species. They also provide a framework upon which sequences from similar organisms can be mapped and assembled. Large and complex genomes were difficult or impossible to construct prior to the introduction of third-generation technology. Sanger sequencing was used to determine the sequences of small genomes, however, that approach requires the burdensome, time consuming, and expensive technique of primer walking. Next-generation sequencing (NGS) technology produces short reads that are difficult to assemble. The creation of reference quality genomes with these technologies, especially with very large and\/or polyploid genomes, presents often insurmountable challenges.<\/p>\n

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