{"id":21413,"date":"2021-06-01T22:39:40","date_gmt":"2021-06-02T05:39:40","guid":{"rendered":"https:\/\/www.novogene.com\/us-en\/?page_id=21413"},"modified":"2025-04-08T16:07:53","modified_gmt":"2025-04-08T23:07:53","slug":"16s-18s-its-amplicon-metagenomic-sequencing","status":"publish","type":"page","link":"https:\/\/www.novogene.com\/us-en\/services\/research-services\/metagenome-sequencing\/16s-18s-its-amplicon-metagenomic-sequencing\/","title":{"rendered":"16S\/18S\/ITS Amplicon Metagenomic Sequencing"},"content":{"rendered":"
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Service Specifications<\/a><\/div>\n<\/div>\n<\/div>\n

16S Amplicon Metagenomic Sequencing, 18S Amplicon Metagenomic Sequencing, and 16S\/18S\/ITS Amplicon Metagenomic Sequencing<\/strong>\u00a0are ultra-deep DNA sequencing method that focuses on sequencing specific target regions\u00a0(amplicons)<\/strong>. Short (<500 bp) hypervariable regions of conserved genes or intergenic regions are amplified by PCR and analyzed by\u00a0next generation sequencing (NGS)<\/strong>\u00a0technology, to identify and differentiate multiple microbial species from complicated samples.
\nAmplicon sequencing is designated to sequence the target genes of\u00a016S ribosomal RNA (rRNA)<\/strong>, or\u00a018S rRNA<\/strong>\u00a0and\u00a0Internal Transcribed Spacer (ITS) rRNA<\/strong>\u00a0by universal primers. Through utilizing 16S rRNA gene sequencing, 18S rRNA gene sequencing, and ITS rRNA gene sequencing, research can describe and compare the phylogeny and taxonomy of bacteria (and archaea) and fungi (such as yeasts, molds and etc.), respectively.<\/p>\n

Novogene’s amplicon sequencing<\/strong> services provide to efficiently screen for variants and target organisms, and describe and compare the diversity of multiple complex environments. The approach is frequently used in population and community microbial ecology studies, phylogenetic reconstruction of target microbial groups, identification of individual species in pure cultures, and detection of organisms of interest (pathogens or beneficial), among many others.<\/p>\n

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    Resources<\/h1>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n