{"id":36927,"date":"2024-01-21T23:14:32","date_gmt":"2024-01-22T07:14:32","guid":{"rendered":"https:\/\/www.novogene.com\/us-en\/?post_type=news&#038;p=36927"},"modified":"2025-01-09T01:52:31","modified_gmt":"2025-01-09T09:52:31","slug":"novaseq-x-plus-25b-at-novogene-redefining-genomic-sequencing-excellence","status":"publish","type":"news","link":"https:\/\/www.novogene.com\/us-en\/about\/news\/novaseq-x-plus-25b-at-novogene-redefining-genomic-sequencing-excellence\/","title":{"rendered":"NovaSeq X Plus 25B at Novogene: Redefining Genomic Sequencing Excellence"},"content":{"rendered":"<style>\n        p.novofont {\n            line-height: 20px;\n            color: #1f3f5a;\n        }\n        p.fontsize14 {\n            font-size: 14px;\n        }\n        .novo-table {\n            border: 1px solid #e5e5e5;\n            border-collapse: collapse;\n            margin-top: 10px;\n            font-size: 12.87px;\n        }\n        .novo-table tr {\n            border: 1px solid #e5e5e5;\n        }\n        .novo-table tr:nth-child(1) {\n            font-weight: bold;\n        }\n        .novo-table tr td {\n            border: 1px solid #e5e5e5;\n            padding: 10px;\n            text-align: center;\n            width: 200px;\n        }\n        h5 {\n            color: #1f3f5a;\n        }\n        h4 {\n            font-weight: bold;\n        }\n        .center {\n            text-align: center;\n        }\n        .novop {\n            text-indent: 2em;\n            line-height: 30px;\n        }\n        .novo-div {\n            width: 80%;\n            margin: 0 auto;\n        }\n    <\/style>\n<h4 style=\"margin:20px 0;\">Technical Breakdown and Exclusive First Look: Unmatched Precision in Sequencing<\/h4>\n<h2><strong>Introduction<\/strong><\/h2>\n<p>We are thrilled to introduce the NovaSeq X Plus 25B flow cell (FC), a state-of-the-art sequencing platform with unmatched throughput capabilities. The 25B flow cell enables you to step into the future of genomics with Illumina\u2019s latest innovation. This remarkable high-density 25B flow cell empowers your research with unparalleled capabilities. <\/p>\n<h2><strong>Unprecedented High Throughput Potential of the 25B FC<\/strong><\/h2>\n<p>The NovaSeq X Plus System, loaded with 25B FC offers the unmatched throughput and accuracy needed to enable more data-intensive applications and deliver the most cost-effective solution. The NovaSeq X Plus is Illumina\u2019s most powerful sequencing system yet, capable of generating up to 16 terabases (TB) of output (or up to 52 billion single reads) per dual 25B flow cell run. This represents a remarkable 2.5x increase from the 10B FC and an incredible 3x increase from the S4 FC (Table 1). <\/p>\n<p>The performance of the NovaSeq X Series reduces the cost per gigabase (Gb) by up to 60% compared to the NovaSeq 6000 System [1]. With the cutting-edge NovaSeq X Plus 25B sequencing, you can enjoy the most cost-effective sequencing service for your research.<\/p>\n<table class=\"novo-table\">\n<tr>\n<td>\u00a0<\/td>\n<td><strong>NovaSeq X Plus 25B<\/strong><\/td>\n<td><strong>NovaSeq X Plus 10B<\/strong><\/td>\n<td><strong>NovaSeq 6000\u00a0S4\u00a0<\/strong><\/td>\n<\/tr>\n<tr>\n<td>Flow Cells\u00a0per Run<\/td>\n<td>2<\/td>\n<td>2<\/td>\n<td>2<\/td>\n<\/tr>\n<tr>\n<td>Lanes per FC<\/td>\n<td>8<\/td>\n<td>8<\/td>\n<td>4<\/td>\n<\/tr>\n<tr>\n<td>Sequencing Mode Available at Novogene<\/td>\n<td colspan=\"3\">PE150\u00a0<\/td>\n<\/tr>\n<tr>\n<td>Theoretical Data Output per Lane (PE 150)\u00a0<\/td>\n<td>1000 Gb3.3B paired reads<\/td>\n<td>375 Gb1.25B paired reads<\/td>\n<td>800 Gb\u00a02.7B paired reads<\/td>\n<\/tr>\n<tr>\n<td>Chemistry for Patterned Flow Cells\u00a0<\/td>\n<td colspan=\"2\">XLEAP-SBS Chemistry\u00a02x faster cycle time\u00a03x accuracy increase [1]<\/td>\n<td>SBS\u00a0Chemistry<\/td>\n<\/tr>\n<tr>\n<td>Channel Chemistry\u00a0<\/td>\n<td colspan=\"2\">2-channel chemistry (blue-green optics)\u00a0<\/td>\n<td>2-channel chemistry (red-green optics)\u00a0<\/td>\n<\/tr>\n<tr>\n<td>Quality at PE150\u00a0<\/td>\n<td colspan=\"2\">\u2265 85% of bases higher than Q30 <\/td>\n<td>\u2265 85% of bases higher than Q30\u00a0<\/td>\n<\/tr>\n<tr>\n<td>Acceptable Premade Library Type\u00a0<\/td>\n<td colspan=\"3\">Broad library types with Illumina compatibility <\/td>\n<\/tr>\n<\/table>\n<p>Table 1: Technical Comparison of Sequencing Platforms: NovaSeq X Plus 10B vs. NovaSeq X Plus 25B vs. NovaSeq 6000 S4<\/p>\n<h2><strong>Unmatched Precision with 25B<\/strong><\/h2>\n<p>In our initial evaluation using a single 25B FC with diverse in-house and client library samples, we observed remarkable data output surpassing 10T, averaging 1200 Gb per lane (Table 2). Notably, the majority of in-house library samples showcased exceptional data quality, with approximately 96% of bases achieving quality scores above Q30. For comparison, both NovaSeq 6000 S4 and X Plus have an established Q30 benchmark of 85%.<\/p>\n<table class=\"novo-table\">\n<tr>\n<td><strong>Type<\/strong><\/td>\n<td><strong>Lane 1<\/strong><\/td>\n<td><strong>Lane 2<\/strong><\/td>\n<td><strong>Lane 3<\/strong><\/td>\n<td><strong>Lane 4<\/strong><\/td>\n<td><strong>Lane 5<\/strong><\/td>\n<td><strong>Lane 6<\/strong><\/td>\n<td><strong>Lane 7<\/strong><\/td>\n<td><strong>Lane 8<\/strong><\/td>\n<\/tr>\n<tr>\n<td>\n<p>Library Type<\/p>\n<\/td>\n<td>\n<p>WES<\/p>\n<p>Metagenomic<\/p>\n<p>WGS<\/p>\n<p>ChIP<\/p>\n<p>mRNA<\/p>\n<p>Strand-specific mRNA<\/p>\n<p>Metatranscriptome<\/p>\n<p>lncRNA<\/p>\n<p>circRNA<\/p>\n<\/td>\n<td>\n<p>WES<\/p>\n<p>Metagenomic<\/p>\n<p>WGS<\/p>\n<p>ChIP<\/p>\n<p>mRNA<\/p>\n<p>Strand-specific mRNA<\/p>\n<p>Metatranscriptome<\/p>\n<p>lncRNA<\/p>\n<p>circRNA<\/p>\n<\/td>\n<td>\n<p>Client Plant &amp; Animal WGS<\/p>\n<\/td>\n<td>\n<p>Client Single Cell RNA<\/p>\n<\/td>\n<td>\n<p>WGBS<\/p>\n<p>PCR-Free<\/p>\n<p>Panel<\/p>\n<\/td>\n<td>\n<p>WGBS<\/p>\n<p>PCR-Free<\/p>\n<p>Panel<\/p>\n<\/td>\n<td>\n<p>CUT&amp;TAG<\/p>\n<p>ATAC<\/p>\n<p>Ribo<\/p>\n<p>RIP<\/p>\n<p>RNA methyl<\/p>\n<p>UMI sRNA<\/p>\n<p>UMI mRNA<\/p>\n<p>Hi-C<\/p>\n<p>Exosome lncRNA<\/p>\n<p>10x 3&#8242;<\/p>\n<p>10x 5&#8242;<\/p>\n<\/td>\n<td>\n<p>CUT&amp;TAG<\/p>\n<p>ATAC<\/p>\n<p>Ribo<\/p>\n<p>RIP<\/p>\n<p>RNA methyl<\/p>\n<p>UMI sRNA<\/p>\n<p>UMI mRNA<\/p>\n<p>Hi-C<\/p>\n<p>Exosome lncRNA<\/p>\n<p>10x 3&#8242;<\/p>\n<p>10x 5&#8242;<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td>Data Output \/ Lane (Gb)<\/td>\n<td>1279<\/td>\n<td>1280<\/td>\n<td>1309<\/td>\n<td>1198<\/td>\n<td>1344<\/td>\n<td>1359<\/td>\n<td>1300<\/td>\n<td>1303<\/td>\n<\/tr>\n<tr>\n<td>Data Output\/Lane (Gb) (excluding index reads)<\/td>\n<td>1206<\/td>\n<td>1208<\/td>\n<td>1235<\/td>\n<td>1130<\/td>\n<td>1268<\/td>\n<td>1282<\/td>\n<td>1227<\/td>\n<td>1230<\/td>\n<\/tr>\n<tr>\n<td>Q30<\/td>\n<td>95.85%<\/td>\n<td>95.77%<\/td>\n<td>94.08%<\/td>\n<td>77.99%<\/td>\n<td>95.55%<\/td>\n<td>95.89%<\/td>\n<td>91.7%<\/td>\n<td>91.67%<\/td>\n<\/tr>\n<\/table>\n<p>Table 2: Library Pooling and Configuration for 25B Flow Cell Evaluation\u2212\u2212A single 25B FC was tested using a diverse range of library types<\/p>\n<p>WES: Whole Exome Sequencing;  Metagenomic: Metagenomic Library; WGS: Whole Genome Sequencing; ChIP: Chromatin Immunoprecipitation Sequencing; mRNA: Eukaryotic mRNA Sequencing; Metatranscriptome: Metatranscriptomic Library; LncRNA: Long Noncoding RNA Sequencing; circRNA: Circular RNA Sequencing; WGBS: Whole Genome Bisulfite Sequencing; PCR-free: PCR-free WGS; Panel: Gene Panel Sequencing; CUT&amp;TAG: Cleavage Under Targets and Tagmentation; ATAC: ATAC-seq; Ribo: Ribosome Profiling; RIP: RNA Immunoprecipitation Sequencing; RNA methyl: RNA Methylation Sequencing; UMI sRNA: UMI Small RNA-seq; UMI mRNA: UMI mRNA-seq; Hi-C: Hi-C seq; 10x 3&#8242;: 10x Genomics Chromium Single Cell 3&#8242; Gene Expression; 10x 5&#8242;: 10x Genomics Chromium Single Cell 5&#8242; Gene Expression.<\/p>\n<p><strong>Platform Variations Decoded<\/strong><\/p>\n<p>We are pleased to present a comprehensive data analysis encompassing our principal sequencing products: Human Whole Genome Sequencing (WGS), Whole Exome Sequencing (WES), Human mRNA Sequencing, Metagenomics Library Sequencing, and Whole Genome Bisulfite Sequencing (WGBS). Our rigorous evaluation involved testing identical samples across three distinct configurations: NovaSeq 6000 S4, NovaSeq X Plus 10B, and NovaSeq X Plus 25B, facilitating a robust comparative assessment of performance across these sequencing methodologies. To access and download the full text analysis and report, please find the link at the bottom of the article.<\/p>\n<p><img decoding=\"async\" alt=\"A group of graphs with blue and red linesDescription automatically generated\" src=\"https:\/\/www.novogene.com\/us-en\/wp-content\/uploads\/sites\/4\/2024\/01\/1705654916.527023.png\" \/><\/p>\n<p class=\"text-center\">Figure: GC Bias Plots of Human WGS Samples<br \/>HU1: Control Sample; HU1-L1 and HU1-L2: Human WGS Samples on 25B Lane 1 or Lane 2; HUP: Control PCR-free Sample; HUP1-L1 and<br \/>HUP1-L2: Human PCR-free WGS Samples on 25B Lane 1 or Lane 2.<\/p>\n<p><strong>Reference<\/strong><\/p>\n<p>[1] Illumina. NovaSeq X and X Plus System specification sheet. <a href=\"https:\/\/www.illumina.com\/content\/dam\/illumina\/gcs\/assembled-assets\/marketing-literature\/novaseq-x-series-spec-sheet-m-us-00197\/novaseq-x-series-specification-sheet-m-us-00197.pdf\">https:\/\/www.illumina.com\/content\/dam\/illumina\/gcs\/assembled-assets\/marketing-literature\/novaseq-x-series-spec-sheet-m-us-00197\/novaseq-x-series-specification-sheet-m-us-00197.pdf<\/a><\/p>\n<p>[2] ENCODE. Whole-Genome Bisulfite Sequencing Data Standards and Processing Pipeline. <a href=\"https:\/\/www.encodeproject.org\/data-standards\/wgbs\/\">https:\/\/www.encodeproject.org\/data-standards\/wgbs\/<\/a><\/p>\n<p><strong>Related Links<\/strong><\/p>\n<p><a href=\"https:\/\/www.novogene.com\/us-en\/resources\/brochure\/novaseq-x-plus-25b-at-novogene-redefining-genomic-sequencing-excellence\/\">Comprehensive 25B Test Report with Supplementary Figures<\/a><\/p>\n<p><a href=\"https:\/\/web.novogene.com\/Website_NovaSeqXPlus25B_PremadeLibrarySeq\">Early Bird Special: NovaSeg X Plus 25B for Pre-made Library Sequencing Service<\/a><\/p>\n","protected":false},"featured_media":37377,"parent":0,"menu_order":8,"template":"","meta":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v20.8 (Yoast SEO v20.8) - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>NovaSeq X Plus 25B at Novogene: Redefining Genomic Sequencing Excellence - Novogene<\/title>\n<meta name=\"description\" content=\"-\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.novogene.com\/us-en\/about\/news\/novaseq-x-plus-25b-at-novogene-redefining-genomic-sequencing-excellence\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"NovaSeq X Plus 25B at Novogene: Redefining Genomic Sequencing Excellence\" \/>\n<meta property=\"og:description\" content=\"-\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.novogene.com\/us-en\/about\/news\/novaseq-x-plus-25b-at-novogene-redefining-genomic-sequencing-excellence\/\" \/>\n<meta property=\"og:site_name\" content=\"Novogene\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/NovogeneAmerica\/\" \/>\n<meta property=\"article:modified_time\" content=\"2025-01-09T09:52:31+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.novogene.com\/us-en\/wp-content\/uploads\/sites\/4\/2024\/01\/1705654916.527023-1.png\" \/>\n\t<meta property=\"og:image:width\" content=\"1802\" \/>\n\t<meta property=\"og:image:height\" content=\"1252\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:site\" content=\"@Novogene_Global\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"5 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.novogene.com\/us-en\/about\/news\/novaseq-x-plus-25b-at-novogene-redefining-genomic-sequencing-excellence\/\",\"url\":\"https:\/\/www.novogene.com\/us-en\/about\/news\/novaseq-x-plus-25b-at-novogene-redefining-genomic-sequencing-excellence\/\",\"name\":\"NovaSeq X Plus 25B at Novogene: Redefining Genomic Sequencing Excellence - 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