{"id":38682,"date":"2026-04-14T00:46:03","date_gmt":"2026-04-14T07:46:03","guid":{"rendered":"https:\/\/www.novogene.com\/eu-en\/?post_type=resources&p=38682"},"modified":"2026-04-16T00:49:29","modified_gmt":"2026-04-16T07:49:29","slug":"plasmidgo-beginners-guide-ont-based-whole-plasmid-sequencing","status":"publish","type":"resources","link":"https:\/\/www.novogene.com\/eu-en\/resources\/onlineevent\/plasmidgo-beginners-guide-ont-based-whole-plasmid-sequencing\/","title":{"rendered":"PlasmidGo! Beginner\u2019s Guide: ONT-based Whole Plasmid Sequencing"},"content":{"rendered":"
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Introduction:<\/strong> <\/p>\n

Since the late 1970s, plasmids have been one of the most widely used tools for genetic engineering and expression. Plasmid validation has long relied on Sanger sequencing as the gold standard. But as synthetic biology and gene therapy push the boundaries of complexity, traditional approaches are falling short.<\/p>\n

The Challenge: Fragmented data, laborious “primer walking,” and invisible errors in repetitive regions or ITRs.<\/p>\n

The Transition to Whole Plasmid Sequencing: Oxford Nanopore Technologies (ONT) long-read sequencing enables continuous coverage across entire plasmids, allowing both insert and backbone to be verified in a single workflow.<\/p>\n

What You Will Learn<\/strong><\/p>\n

This beginner-friendly session explores the transition from fragmented traditional methods to next-generation plasmid validation:<\/p>\n