{"id":37000,"date":"2024-06-03T23:57:24","date_gmt":"2024-06-04T06:57:24","guid":{"rendered":"https:\/\/www.novogene.com\/eu-en\/?page_id=37000"},"modified":"2025-03-31T02:54:53","modified_gmt":"2025-03-31T09:54:53","slug":"full-length-16s-18s-its-amplicon-sequencing","status":"publish","type":"page","link":"https:\/\/www.novogene.com\/eu-en\/services\/research-services\/metagenome-sequencing\/full-length-16s-18s-its-amplicon-sequencing\/","title":{"rendered":"Full-Length 16S\/18S\/ITS Amplicon Sequencing"},"content":{"rendered":"<div class=\"clear_p show_p_img\" style=\"float: right;margin: 0 0 20px 50px\">\n<img decoding=\"async\" class=\"clear_img\" style=\"width: 286px\" src=\"https:\/\/www.novogene.com\/eu-en\/wp-content\/uploads\/sites\/7\/2021\/06\/novogene-istockphoto.jpg\" alt=\"\" \/><\/p>\n<div class=\"col-sm-12 m-auto text-center\" style=\"padding-top: 20px;padding-left: 0\">\n<span style=\"float: left;background: #3f6992;height: 2.6875rem;width: 2.875rem\"><img decoding=\"async\" style=\"margin-top: 0.25rem\" src=\"https:\/\/www.novogene.com\/eu-en\/wp-content\/uploads\/sites\/7\/2021\/01\/Novogene-button-icon-20210129.png\" \/><\/span><\/p>\n<div id=\"top_btn\" class=\"blue btn\" style=\"margin-top: 0px;padding: 8px 1.1875rem;float: left;background: #3789C7;border: none;line-height: 1.6875rem;width:241px;\"><a class=\"\" href=\"https:\/\/www.novogene.com\/eu-en\/wp-content\/uploads\/sites\/7\/2024\/06\/2024-Microbial-WGS-and-metagenomics-flyer-updated-4.pdf\">Service Flyer<\/a><\/div>\n<\/div>\n<\/div>\n<p class=\"blue-box\" style=\"width: 70%;background-color: #e2f2ff!important;padding: 20px;font-family: 'Montserrat-Regular', Arial;color: #032e53;font-size: 16px\"><strong>Full-Length 16S\/18S\/ITS Amplicon Sequencing<\/strong> is a metagenomic approach that delves deep into the taxonomic composition of complex microbial communities. This sophisticated method amplifies full-length conserved genes, including 16S ribosomal RNA (rRNA), 18S rRNA, and Internal Transcribed Spacer (ITS) rRNA, using PCR techniques. The resulting amplicons are then subjected to high-fidelity, long-read sequencing on the <strong>PacBio Revio<\/strong> platform.<\/p>\n<p style=\"line-height: 24px\">Full-length amplicon sequencing provides unparalleled taxonomic resolution. Unlike traditional methods that focus solely on hypervariable regions or intergenic regions, full-length amplicon sequencing captures the entire gene sequence. These amplicons range in length from 1-2kb, lengths that short-read sequencing cannot fully capture. This comprehensive approach reveals a richer, more detailed picture of microbial diversity, allowing for more accurate identification and classification of species within complex samples. Our <strong>16S rRNA pipeline utilises PacBio\u2019s Kinnex 16S rRNA<\/strong> library preparation kit to further improve accuracy and throughput.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Service Flyer Full-Length 16S\/18S\/ITS Amplicon Sequencing is a metagenomic approach that delves deep into the taxonomic composition of complex microbial communities. This sophisticated method amplifies full-length conserved genes, including 16S ribosomal RNA (rRNA), 18S rRNA, and Internal Transcribed Spacer (ITS) rRNA, using PCR techniques. The resulting amplicons are then subjected to high-fidelity, long-read sequencing on<\/p>\n","protected":false},"author":14,"featured_media":0,"parent":24599,"menu_order":20,"comment_status":"closed","ping_status":"closed","template":"templates\/page-technical3.php","meta":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v20.8 (Yoast SEO v20.8) - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Full-Length 16S\/18S\/ITS Amplicon Sequencing - Novogene<\/title>\n<meta name=\"description\" content=\"16S\/18S\/ITS Amplicon Metagenomic Sequencing is an ultra-deep DNA sequencing method that focuses on sequencing specific target regions (amplicons).\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.novogene.com\/eu-en\/services\/research-services\/metagenome-sequencing\/full-length-16s-18s-its-amplicon-sequencing\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Full-Length 16S\/18S\/ITS Amplicon Sequencing\" \/>\n<meta property=\"og:description\" content=\"16S\/18S\/ITS Amplicon Metagenomic Sequencing is an ultra-deep DNA sequencing method that focuses on sequencing specific target regions (amplicons).\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.novogene.com\/eu-en\/services\/research-services\/metagenome-sequencing\/full-length-16s-18s-its-amplicon-sequencing\/\" \/>\n<meta property=\"og:site_name\" content=\"Novogene\" \/>\n<meta property=\"article:modified_time\" content=\"2025-03-31T09:54:53+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.novogene.com\/eu-en\/wp-content\/uploads\/sites\/7\/2021\/06\/novogene-istockphoto.jpg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.novogene.com\/eu-en\/services\/research-services\/metagenome-sequencing\/full-length-16s-18s-its-amplicon-sequencing\/\",\"url\":\"https:\/\/www.novogene.com\/eu-en\/services\/research-services\/metagenome-sequencing\/full-length-16s-18s-its-amplicon-sequencing\/\",\"name\":\"Full-Length 16S\/18S\/ITS Amplicon Sequencing - 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