{"id":24497,"date":"2021-09-17T23:14:25","date_gmt":"2021-09-18T06:14:25","guid":{"rendered":"https:\/\/novogene.com\/eu-en\/?page_id=24497"},"modified":"2025-03-31T02:50:17","modified_gmt":"2025-03-31T09:50:17","slug":"reduced-representation-bisulfite-sequencing-rrbs","status":"publish","type":"page","link":"https:\/\/www.novogene.com\/eu-en\/services\/research-services\/epigenome-sequencing\/reduced-representation-bisulfite-sequencing-rrbs\/","title":{"rendered":"Reduced Representation Bisulfite Sequencing (RRBS)"},"content":{"rendered":"
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Service Flyer<\/a><\/div>\n<\/div>\n<\/div>\n

Reduced Representation Bisulfite Sequencing (RRBS) is a technique used to study DNA methylation which focuses on CpG islands and other CpG-rich regions of the genome. Novogene\u2019s method uses the methylation insensitive restriction enzyme MspI which cuts DNA at CpG-rich sites, regions that are the most common sites of methylation in mammalian species. After enriching for CpG rich regions, DNA undergoes bisulfite treatment which converts the unmethylated cytosines to uracil, while methylated cytosines remain unchanged. Subsequently, we sequence the DNA to determine the methylation status of cytosines by comparing treated DNA to the reference genome.<\/p>\n

RRBS is considered a cost-effective method for DNA methylation analysis, as it reduces the amount of sequencing data needed by focusing only on regions of interest such as CpG islands, thus making it suitable for large-scale studies. If you are interested in analysing methylation across the entire genome, including both CpG and non-CpG contexts, please refer to Whole Genome Methylation Sequencing<\/a>. <\/p>\n