{"id":20731,"date":"2021-05-25T02:43:01","date_gmt":"2021-05-25T09:43:01","guid":{"rendered":"https:\/\/novogene.com\/eu-en\/?page_id=20731"},"modified":"2025-05-13T01:32:00","modified_gmt":"2025-05-13T08:32:00","slug":"mrna-sequencing","status":"publish","type":"page","link":"https:\/\/www.novogene.com\/eu-en\/services\/research-services\/transcriptome-sequencing\/mrna-sequencing\/","title":{"rendered":"mRNA Sequencing (mRNA-seq)"},"content":{"rendered":"<div class=\"clear_p\" style=\"float: right; margin: 0 0 20px 50px;\">\n<img decoding=\"async\" class=\"clear_img\" style=\"width: 286px; min-height: 280px;\" src=\"https:\/\/www.novogene.com\/eu-en\/wp-content\/uploads\/sites\/7\/2020\/04\/istockphoto-1157314129-540x360-2.jpg\" alt=\"\" \/><\/p>\n<div class=\"col-sm-12 m-auto text-center\" style=\"padding-top: 20px; padding-left: 0;\">\n<span style=\"float: left; background: #3f6992; height: 2.6875rem; width: 2.875rem;\"><img decoding=\"async\" style=\"margin-top: 0.25rem;\" src=\"https:\/\/www.novogene.com\/eu-en\/wp-content\/uploads\/sites\/7\/2021\/01\/Novogene-button-icon-20210129.png\" \/><\/span><\/p>\n<div id=\"top_btn\" class=\"blue btn\" style=\"margin-top: 0px; padding: 8px 1.1875rem; float: left; background: #3789C7; border: none; line-height: 1.6875rem;width:241px;\"><a class=\"\" href=\"https:\/\/www.novogene.com\/eu-en\/wp-content\/uploads\/sites\/7\/2024\/07\/2024-Eukaryotic-mRNA-seq-flyer.pdf\">Service Flyer<\/a><\/div>\n<\/div>\n<\/div>\n<p class=\"blue-box\" style=\"width: 70%; background-color: #e2f2ff!important; padding: 20px; font-family: 'Montserrat-Regular', Arial; color: #032e53; font-size: 16px;\"><strong>RNA sequencing (RNA-seq)<\/strong> revolutionizes the understanding of cellular functions, which provides researchers with an unprecedented insight into the transcriptional landscape of cells. Employing the high-throughput and accurate <strong>next-generation sequencing (NGS) technique<\/strong>, RNA-seq reveals <strong>gene expression<\/strong> profiles and describes the <strong>continuous variations<\/strong> in the transcriptome. In the RNA-seq technique, the single-stranded <strong>messenger RNAs (mRNAs)<\/strong> are selectively captured or enriched and converted to <strong>complementary DNA (cDNA)<\/strong> for library preparation.<\/p>\n<p style=\"line-height: 24px;\">At Novogene, cDNA libraries are sequenced using the state-of-the-art <a href=\"https:\/\/www.novogene.com\/eu-en\/technology\/platforms\/\">Illumina NovaSeq platforms<\/a>, which utilizes a paired-end 150 bp sequencing strategy (short-reads) and can be applied to a wide array of research objectives. In addition to eukaryotic <strong>mRNA sequencing (mRNA-seq)<\/strong>, we can deliver data on <a href=\"https:\/\/www.novogene.com\/eu-en\/services\/research-services\/prokaryote\/prokaryotic-rna-sequencing\/\">prokaryotic transcripts<\/a>, <a href=\"https:\/\/www.novogene.com\/eu-en\/services\/research-services\/transcriptome-sequencing\/non-coding-rna-sequencing\/\">non-coding RNAs<\/a>, <a href=\"https:\/\/www.novogene.com\/eu-en\/services\/research-services\/transcriptome-sequencing\/isoform-sequencing-service-iso-seq\/\">full-length isoforms<\/a> (long-reads), <a href=\"https:\/\/www.novogene.com\/eu-en\/services\/research-services\/transcriptome-sequencing\/whole-transcriptome-sequencing\/\">whole transcriptomes<\/a> and <a href=\"https:\/\/www.novogene.com\/eu-en\/services\/research-services\/prokaryote\/metatranscriptome-sequencing\/\">meta-transcriptomes<\/a>.<\/p>\n<style>\n.novo-ul li strong{font-family: 'Merriweather-Bold', Airal !important;}\n.sf_table td{word-break: break-word !important;}\n<\/style>\n","protected":false},"excerpt":{"rendered":"<p>Service Flyer RNA sequencing (RNA-seq) revolutionizes the understanding of cellular functions, which provides researchers with an unprecedented insight into the transcriptional landscape of cells. Employing the high-throughput and accurate next-generation sequencing (NGS) technique, RNA-seq reveals gene expression profiles and describes the continuous variations in the transcriptome. In the RNA-seq technique, the single-stranded messenger RNAs (mRNAs)<\/p>\n","protected":false},"author":10,"featured_media":0,"parent":2120,"menu_order":169,"comment_status":"closed","ping_status":"closed","template":"templates\/page-technical3.php","meta":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v20.8 (Yoast SEO v20.8) - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>mRNA Sequencing (mRNA-seq) - Novogene<\/title>\n<meta name=\"description\" content=\"Novogene mRNA-seq offers high throughput and high accuracy (with Q30 score \u2265 85%) coupled with a low initial RNA input required.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.novogene.com\/eu-en\/services\/research-services\/transcriptome-sequencing\/mrna-sequencing\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"mRNA Sequencing (mRNA-seq)\" \/>\n<meta property=\"og:description\" content=\"Novogene mRNA-seq offers high throughput and high accuracy (with Q30 score \u2265 85%) coupled with a low initial RNA input required.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.novogene.com\/eu-en\/services\/research-services\/transcriptome-sequencing\/mrna-sequencing\/\" \/>\n<meta property=\"og:site_name\" content=\"Novogene\" \/>\n<meta property=\"article:modified_time\" content=\"2025-05-13T08:32:00+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.novogene.com\/eu-en\/wp-content\/uploads\/sites\/7\/2020\/04\/istockphoto-1157314129-540x360-2.jpg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.novogene.com\/eu-en\/services\/research-services\/transcriptome-sequencing\/mrna-sequencing\/\",\"url\":\"https:\/\/www.novogene.com\/eu-en\/services\/research-services\/transcriptome-sequencing\/mrna-sequencing\/\",\"name\":\"mRNA Sequencing (mRNA-seq) - 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